Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea
Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea
复制标题
DOI：
10.1105/tpc.105.032961
复制
发表时间：
2005-07-01
期刊：
影响因子：
11.6
通讯作者：
Murata, M
中科院分区：
文献类型：
--
作者：
Nagaki, K;Kashihara, K;Murata, M
虽然全新中心物种分散在植物和动物王国中，但用着丝粒蛋白标记分析了线虫线虫的全新中心染色体。为了确定植物的全新中心结构，我们对雪木杉进行了研究。基于植物CENH3s序列的相似性，成功地从幼根中分离出了一个推定为着丝粒特异性组蛋白H3 （LnCENH3）的cDNA。然后用推导出的氨基酸序列培养抗lncenh3抗体。免疫染色清楚地显示弥漫性着丝粒样结构，在前期到末期呈线状。此外，间期LnCENH3的表达量明显减少。每条染色单体在中期到后期的极侧定位也证实了LnCENH3是乳杆菌着丝粒特异性蛋白之一。将这些数据与秀丽隐杆线虫的数据进行了比较，并讨论了全新中心染色体的共同特征。
Although holocentric species are scattered throughout the plant and animal kingdoms, only holocentric chromosomes of the nematode worm Caenorhabditis elegans have been analyzed with centromeric protein markers. In an effort to determine the holocentric structure in plants, we investigated the snowy woodrush Luzula nivea. From the young roots, a cDNA encoding a putative centromere-specific histone H3 (LnCENH3) was successfully isolated based on sequence similarity among plant CENH3s. The deduced amino acid sequence was then used to raise an anti-LnCENH3 antibody. Immunostaining clearly revealed the diffuse centromere-like structure that appears in the linear shape at prophase to telophase. Furthermore, it was shown that the amount of LnCENH3 decreased significantly at interphase. The polar side positioning on each chromatid at metaphase to anaphase also confirmed that LnCENH3 represents one of the centromere-specific proteins in L. nivea. These data from L. nivea are compared with those from C. elegans, and common features of holocentric chromosomes are discussed.