Effects of Aspirin on Clot Structure and Fibrinolysis Using a Novel In Vitro Cellular System
Effects of Aspirin on Clot Structure and Fibrinolysis Using a Novel In Vitro Cellular System
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DOI：
10.1161/atvbaha.109.183707
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发表时间：
2009-05-01
影响因子：
8.7
通讯作者：
Grant, P. J.
中科院分区：
文献类型：
--
作者：
Ajjan, R. A.;Standeven, K. F.;Grant, P. J.
目的：探讨阿司匹林对纤维蛋白结构/功能的直接影响。方法与结果：在不加(0)和加浓度阿司匹林的情况下，稳定转染纤维蛋白原的中国仓鼠卵巢细胞系生长。用亲和层析法从培养基中纯化纤维蛋白原，并用重组蛋白制备凝块。由0、1、10和100 mg/L阿司匹林处理的纤维蛋白原制成的血块的平均最终浊度[OD(+/- SEM)]分别为0.083（+/- 0.03）、0.093（+/- 0.002）、0.101（+/- 0.005）和0.125（+/- 0.003）
Objectives-The purpose of this study was to investigate the direct effects of aspirin on fibrin structure/function.Methods and Results-Chinese Hamster Ovary cell lines stably transfected with fibrinogen were grown in the absence ( 0) and presence of increasing concentrations of aspirin. Fibrinogen was purified from the media using affinity chromatography, and clots were made from recombinant protein. Mean final turbidity [OD(+/- SEM)] was 0.083(+/- 0.03), 0.093(+/- 0.002), 0.101(+/- 0.005), and 0.125(+/- 0.003) in clots made from 0, 1, 10, and 100 mg/L aspirin-treated fibrinogen, respectively (P