Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa
Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa
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DOI：
10.1530/rep.1.00531
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发表时间：
2005-04-01
期刊：
影响因子：
3.8
通讯作者：
Saunders, PTK
中科院分区：
文献类型：
--
作者：
Banks, S;King, SA;Saunders, PTK
阴囊温度升高会导致精子质量下降和不育。在本研究中，我们使用小鼠来研究轻度阴囊热应激（42摄氏度，30分钟）对精子数量及其DNA完整性的影响。使用COMET和精子染色质结构（SCSA）测定分析处理后小时（11至24）或天（7至32）从附睾中回收的精子。该处理诱导了睾丸和附睾中的应激反应，这与冷诱导RNA结合蛋白（Cirp）的表达减少和生殖细胞凋亡（Apotag阳性细胞）的增加有关。虽然加热时附睾中的精子含有正确包装的DNA，但其完整性受到热应激的损害。此外，尽管在热应激时存在于睾丸内的一些生殖细胞通过细胞凋亡而被去除，但许多生殖细胞完成了它们的发育并作为具有受损DNA的活动精子而恢复。总之，这些数据表明，阴囊热应激可损害精子的DNA完整性，这可能对接受IVF和胞质内单精子注射（ICSI）的患者具有临床意义。
An increase in scrotal temperature can lead to the production of poor quality spermatozoa and infertility. In the present study we have used mice to examine the impact of mild, scrota[ heat stress (42 degrees C for 30 min) on numbers of spermatozoa as well as on the integrity of their DNA. Spermatozoa recovered from the epididymides hours (11 to 24) or days (7 to 32) after treatment were analysed using COMET and sperm chromatin structure (SCSA) assays. The treatment induced a stress response in both the testis and the epididymis that was associated with reduced expression of the cold inducible RNA binding protein (Cirp) and an increase in germ cell apoptosis (Apotag positive cells). Although spermatozoa present in the epididymis at the time of heating contained correctly packaged DNA, its integrity was compromised by heat stress. in addition, although some germ cells, which were present within the testis at the time of heat stress, were removed by apoptosis, many germ cells completed their development and were recovered as motile spermatozoa with damaged DNA. In conclusion, these data demonstrate that scrotal heat stress can compromise the DNA integrity of spermatozoa and this may have clinical implications for patients undergoing IVF and intra-cytoplasmic sperm injection (ICSI).