Orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy.
Orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy.
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DOI：
10.3791/1989
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发表时间：
2010-12-04
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影响因子：
--
通讯作者：
Schrepfer, Sonja
中科院分区：
文献类型：
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作者：
Stubbendorff, Mandy;Deuse, Tobias;Schrepfer, Sonja
慢性排斥反应的研究模型对于研究移植血管病变（TVP）发展过程中的病理生物学和病理生理过程至关重要。心血管慢性排斥研究常用的动物模型是在实验室啮齿动物中进行的异位心脏移植模型。自从小野和林赛发表他们的技术以来，这个模型在实验中被广泛使用。为了分析血管中的发现，必须对心脏进行切片并测量所有血管。研究心血管问题中慢性排斥反应的另一种方法是主动脉移植模型。在原位主动脉移植模型中，可以轻松地对主动脉进行组织学评估。 PVG-to-ACI 模型对于 CAV 研究特别有用，因为急性血管排斥不是主要的混杂因素，并且环孢素 A (CsA) 治疗不能阻止 CAV 的发展，这与我们在临床环境中发现的情况类似。该模型需要 7 天的 CsA 周期，以防止急性排斥反应并随着 TVP 的发展实现长期生存。该模型还可用于研究异种模型中的急性细胞排斥和介质坏死。
Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant vasculopathy (TVP). The commonly used animal model for cardiovascular chronic rejection studies is the heterotopic heart transplant model performed in laboratory rodents. This model is used widely in experiments since Ono and Lindsey published their technique. To analyze the findings in the blood vessels, the heart has to be sectioned and all vessels have to be measured. Another method to investigate chronic rejection in cardiovascular questionings is the aortic transplant model. In the orthotopic aortic transplant model, the aorta can easily be histologically evaluated. The PVG-to-ACI model is especially useful for CAV studies, since acute vascular rejection is not a major confounding factor and Cyclosporin A (CsA) treatment does not prevent the development of CAV, similar to what we find in the clinical setting. A7-day period of CsA is required in this model to prevent acute rejection and to achieve long-term survival with the development of TVP. This model can also be used to investigate acute cellular rejection and media necrosis in xenogeneic models.