Effects of Lyt antibodies on T-cell functions: augmentation by anti-Lyt-1 as opposed to inhibition by anti-Lyt-2.
Effects of Lyt antibodies on T-cell functions: augmentation by anti-Lyt-1 as opposed to inhibition by anti-Lyt-2.
复制标题
Lyt 抗体对 T 细胞功能的影响：抗 Lyt-1 增强，而不是抗 Lyt-2 抑制。
DOI：
10.1073/pnas.78.2.1148
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发表时间：
1981
影响因子：
11.1
通讯作者：
Weissman,IL
中科院分区：
文献类型：
--
作者：
Hollander,N;Pillemer,E;Weissman,IL
单克隆抗Lyt-1（α Lyt-1）和α Lyt-2在加入不含补体的反应混合物中时，对同种异体混合淋巴细胞反应表现出相反的影响。α Lyt-1增强细胞增殖和细胞毒性细胞的产生，但对细胞介导的细胞溶解没有影响，而α Lyt-2阻断细胞增殖、杀伤细胞的产生和杀伤细胞的细胞溶解活性。α Lyt-1的增强作用不能归因于对T细胞的直接促有丝分裂作用。α Lyt-2的抑制和α Lyt-1的增强都需要在混合淋巴细胞反应的前24小时内应答细胞与抗体相互作用，这表明反应的早期阶段对Lyt抗体敏感。α Lyt-1对同种异体抗原诱导的T细胞增殖的增强作用与T细胞生长因子的产生增加有关。当α Lyt-1存在于混合淋巴细胞培养物中时，培养24或48小时后收集的上清液培养基与在不存在抗体或存在对生长因子分泌没有影响的α Lyt-2的情况下孵育的混合淋巴细胞培养物的培养基相比诱导更高的活化T细胞增殖。α Lyt-1和α Lyt-2的作用差异不能归因于重链恒定区功能的差异，因为两者属于相同的λ 2A免疫球蛋白类别，并且以相同的浓度使用。这些数据表明Lyt-1分子在早期激活和有丝分裂过程中可能发挥作用，如生长因子的产生。
Monoclonal anti-Lyt-1 (alpha Lyt-1) and alpha Lyt-2 manifest inverse effects on allogeneic mixed lymphocyte reactions when added to the reaction mixtures without complement. alpha Lyt-1 augments cell proliferation and generation of cytotoxic cells but has no effect on cell-mediated cytolysis, whereas alpha Lyt-2 blocks cell proliferation, generation of killer cells, and cytolytic activity of killer cells. The augmenting effect of alpha Lyt-1 cannot be attributed to a direct mitogenic effect on T cells. Both inhibition by alpha Lyt-2 and potentiated by alpha Lyt-1 require interaction of responder cells with the antibodies during the first 24 hr of the mixed lymphocyte reaction, indicating that early stages of the reaction are sensitive to Lyt antibodies. The enhancing effect of alpha Lyt-1 on alloantigen-induced T-cell proliferation is associated with augmented production of T-cell growth factors. When alpha Lyt-1 is present in mixed lymphocyte cultures, the supernatant media collected after 24 or 48 hr of culture induce higher proliferation of activated T cells compared to media of mixed lymphocyte cultures incubated in the absence of antibodies or in the presence of alpha Lyt-2 which has no effect on secretion of growth factors. The differences in the effects of alpha Lyt-1 and alpha Lyt-2 could not be attributed to differences in heavy chain constant region functions because both were of the same lambda 2A immunoglobulin class and were used at the same concentration. The data suggest a possible role for Lyt-1 molecules in early activation and mitogenesis processes such as production of growth factors.