POTENTIAL ONCOGENIC EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR REQUIRES COOPERATION BETWEEN CUG AND AUG-INITIATED FORMS
POTENTIAL ONCOGENIC EFFECTS OF BASIC FIBROBLAST GROWTH-FACTOR REQUIRES COOPERATION BETWEEN CUG AND AUG-INITIATED FORMS
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DOI：
10.1091/mbc.2.9.709
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发表时间：
1991-09-01
期刊：
影响因子：
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通讯作者：
AMALRIC, F
中科院分区：
文献类型：
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作者：
COUDERC, B;PRATS, H;AMALRIC, F
用不同的重组逆转录病毒感染正常成年牛主动脉内皮细胞，这些逆转录病毒表达一个、两个或三个人碱性成纤维细胞生长因子(BFGF)蛋白，通常由翻译起始密码子的替代合成。我们在这里表明，AUG-起始自(18 KDa)的结构性表达导致转基因细胞在软琼脂中形成集落。在两个CUG起始密码子之一开始表达的高摩尔重量(HMW)形式(22.5和21 kDa)允许细胞永生化，而当这三种形式被结构性表达时，就达到了致瘤的潜力。此外，我们提供的证据表明，(HMW)bFGF型的结构性表达对亲代内皮细胞中天然活性基因bFGFs的合成具有下调作用。
Normal adult bovine aortic endothelial cells were infected with various recombinant retroviruses expressing one, two, or three human basic fibroblast growth factor (bFGF) proteins normally synthesized by an alternative use of translation initiation codons. We show here that the constitutive expression of the AUG-initiated from (18 kDa) leads the transfected cells to form colonies in soft agar. The expression of the high molar weight (HMW) forms (22.5 and 21 kDa) initiated at one of the two CUG initiation codons allows cell immortalization, whereas the tumorigenic potential is reached when the three forms are constitutively expressed. Furthermore, we provide evidence that constitutive expression of (HMW) bFGF forms has a down-regulation effect on bFGF synthesis from the gene naturally active in parental endothelial cells.