Protein Quantity and Quality of Safflower Seed Improved by NP Fertilizer and Rhizobacteria (Azospirillum and Azotobacter spp.).
Protein Quantity and Quality of Safflower Seed Improved by NP Fertilizer and Rhizobacteria (Azospirillum and Azotobacter spp.).
复制标题
NP肥料和根瘤菌改善红花种子的蛋白质数量和质量（Azospirillum和Azotobacter spp。）。
DOI：
10.3389/fpls.2016.00104
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发表时间：
2016
影响因子：
5.6
通讯作者：
Naz R
中科院分区：
文献类型：
--
作者：
Nosheen A;Bano A;Yasmin H;Keyani R;Habib R;Shah ST;Naz R
根瘤菌（Azotobacter spp）提高了红花种子蛋白的质量和数量。SDS-PAGE分析证实了大豆的蛋白质品质，并发现了对不同根菌组合和低剂量肥料有响应的新条带。PGPR的应用将肥料的使用量减少了50%。蛋白质是人类饮食的重要组成部分。本试验旨在通过植物促生根瘤菌（PGPR）与常规氮磷肥（NP）配合施用，提高红花种子蛋白质品质。以两种红花品种Thori和Saif-32为材料，分别接种固氮螺旋菌和固氮杆菌，在大田条件下进行培养。通过粗蛋白质、氨基酸分析和SDS-PAGE测定蛋白质含量和质量。固氮菌添加1 / 4剂量的肥料（BTQ）可显著提高种子粗蛋白质和氨基酸（蛋氨酸、苯丙氨酸和谷氨酸）（55 ~ 1250%），P≤0.05。BTQ和BTH（偶氮菌加半量肥）分别在Thori和Saif-32中诱导出额外的蛋白条带。偶氮螺旋菌配施半量肥（SPH）和BTQ均能提高红花叶片中吲哚乙酸（IAA）含量（90%）和赤霉素酸（GA）含量（23-27%）。综上所述，氮螺旋菌和固氮杆菌在显著减少（最多75%）NP肥料用量的情况下可以提高红花种子蛋白质的质量和数量。
Rhizobacteria (Azotobacter spp.) have improved the quality and quantity of safflower seed protein. Protein quality was confirmed by SDS-PAGE and new bands were found in response to different combinations of rhizobacteria and lower doses of fertilizers. The PGPR application has reduced the use of fertilizers upto 50%. HIGHLIGHTS Protein is an essential part of the human diet. The aim of this present study was to improve the protein quality of safflower seed by the application of plant growth promoting rhizobacteria (PGPR) in combination with conventional nitrogen and phosphate (NP) fertilizers. The seeds of two safflower cultivars Thori and Saif-32, were inoculated with Azospirillum and Azotobacter and grown under field conditions. Protein content and quality was assessed by crude protein, amino acid analysis, and SDS-PAGE. Seed crude protein and amino acids (methionine, phenylalanine, and glutamic acid) showed significant improvements (55–1250%) by Azotobacter supplemented with a quarter dose of fertilizers (BTQ) at P ≤ 0.05. Additional protein bands were induced in Thori and Saif-32 by BTQ and BTH (Azotobacter supplemented with a half dose of fertilizer) respectively. The Azospirillum in combination with half dose of fertilizer (SPH) and BTQ enhanced both indole acetic acid (IAA) (90%) and gibberellic acid (GA) (23–27%) content in safflower leaf. Taken together, these data suggest that Azospirillum and Azotobacter along with significantly reduced (up to 75%) use of NP fertilizers could improve the quality and quantity of safflower seed protein.