Composition of Sieve-Tube Sap
Composition of Sieve-Tube Sap
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筛管汁液的成分
DOI：
10.1038/1841955a0
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发表时间：
1959
期刊：
影响因子：
64.8
通讯作者：
P. Weatherley
中科院分区：
文献类型：
--
作者：
A. J. Peel;P. Weatherley
如 Kennedy 和 Mittler1 和 us2 所描述，使用柳蚜的口器从柳柳 Salix viminalis 的筛管中获得 SAP。 Mittler3 发现，蔗糖构成了总固体的主要成分，因此对于常规浓度测量，使用直接以蔗糖百分比进行校准的折光仪。通过纸色谱法分析糖含量，使用微量凯氏定氮法4测量总氮含量，并通过纸色谱法检测存在的各个氨基酸。
SAP from the sieve-tubes of the willow Salix viminalis was obtained using the mouthparts of the willow aphid as described by Kennedy and Mittler1 and us2. As Mittler3 found, sucrose formed the main constituent of the total solids and so for routine concentration measurements, a refractometer calibrated directly in percentage sucrose was used. The sugar content was analysed by paper chromato-graphy, the total nitrogen content measured using a micro-kjeldahl method4 and the individual amino-acids present detected by paper chromatography.