Non-invasive diagnosis of H pylori infection: evaluation of serological tests with and without current infection marker CIM.
Non-invasive diagnosis of H pylori infection: evaluation of serological tests with and without current infection marker CIM.
复制标题
幽门螺杆菌感染的无创诊断：使用和不使用当前感染标志物 CIM 的血清学测试评估。
DOI：
10.3748/wjg.14.1231
复制
发表时间：
2008
影响因子：
4.3
通讯作者：
Moatez Hassan
中科院分区：
文献类型：
--
作者：
S. Rahman;MG Azam;Mahmudur Rahman;Misbahul Arfin;M. Alam;T. Bhuiyan;Nasim Ahmed;Motiur Rahman;S. Nahar;Moatez Hassan
目的
评价市售免疫层析（ICT）和免疫印迹试验（涵盖当前感染标志物CIM和常规ELISA）诊断成人消化不良患者幽门螺杆菌感染的性能。
方法
通过培养、快速尿素酶试验和胃活检标本的组织学检查，对接受诊断性内镜检查的非治疗消化不良患者进行幽门螺杆菌感染检测。通过商业ELISA（AccuBind ELISA，Monobind，USA）、ICT（Assure H pylori Rapid Test，Genomics Diagnostics，新加坡）和免疫印迹（Helico Blot 2.1，Genomics Diagnostics，新加坡）测定法，检测来自61名幽门螺杆菌感染患者和21名非感染患者的血清的抗幽门螺杆菌IgG抗体。ICT和免疫印迹试剂盒涵盖CIM以及其他参数，并分别评估了它们在有和没有CIM的情况下的性能。
结果
ELISA的敏感性、特异性、阳性预测值（PPV）、阴性预测值（NPV）和准确性分别为96.7%、42.8%、83.1%、81.8%和82.9%，ICT分别为90.1%、80.9%、93.2%、73.9%和87.8%，ICT联合CIM分别为88.5%、90.4%、96.4%，73.0%、89.0%，免疫印迹法分别为98.3%、80.9%、93.7%、94.4%、93.9%; CIM法分别为98.3%、90.4%、96.7%、95.0%、96.3%。
结论
CIM免疫印迹法性能最好。ICT与CIM联合应用比常规ELISA更具有特异性和准确性，可用于幽门螺杆菌感染的无创性诊断。
AIM
To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in adult dyspeptic patients.
METHODS
Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA (AccuBind ELISA, Monobind, USA), ICT (Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore), and immunoblot (Helico Blot 2.1, Genelabs Diagnostics, Singapore) assays. ICT and immunoblot kits cover CIM among other parameters and their performance with and without CIM was evaluated separately.
RESULTS
Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIM were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIM were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively.
CONCLUSION
Immunoblot with CIM had the best performance. ICT with CIM was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of H pylori infection.
初级保健中消化不良的前瞻性研究。
影响因子：
3.1
作者：
Kurata,JohnH;Nogawa,AkiN;Everhart,JamesE
通讯作者：
Everhart,JamesE