A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans
A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans
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真菌中新的F-肌动蛋白结构：新型隐球菌细胞核周围形成肌动蛋白环
DOI：
10.1093/jmicro/dfs074
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发表时间：
2013
期刊：
影响因子：
1.8
通讯作者：
Marie Kopecka
中科院分区：
文献类型：
--
作者：
Hernandez-Cuellar E;Tsuchiya K;Hara H;Fang R;Sakai S;Kawamura I;Akira S;Mitsuyama M;Marie Kopecka
新型隐球菌的肌动蛋白骨架由肌动蛋白索、皮层片和细胞动力环组成。在这里，我们通过罗丹明鬼笔环肽染色的F-肌动蛋白的荧光显微镜成像，描述了真菌中一种新的F-肌动蛋白结构，即细胞核周围的核周F-肌动蛋白领环。用微管抑制剂诺考达唑或药物溶剂二甲基亚砜（DMSO）处理或在酵母提取物蛋白胨葡萄糖（YEPD）培养基中生长的新生隐球菌中形成核周F-肌动蛋白环，但在用Latrunculin A处理的细胞中不存在。核周F-actin环可能是细胞核的“索舱”，而actin索可能是细胞内的“索”，将细胞核悬挂在细胞的中心位置，并沿着actin索沿极性轴移动。
The F-actin cytoskeleton ofCryptococcus neoformansis known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine phalloidin-stained F-actin. Perinuclear F-actin rings form inCryptococcus neoformanstreated with the microtubule inhibitor Nocodazole or with the drug solvent dimethyl sulfoxide (DMSO) or grown in yeast extract peptone dextrose (YEPD) medium, but they are absent in cells treated with Latrunculin A. Perinuclear F-actin rings may function as ‘funicular cabin’ for the cell nucleus, and actin cables as intracellular ‘funicular’ suspending nucleus in the central position in the cell and moving nucleus along the polarity axis along actin cables.