Thallium transport and the evaluation of olfactory nerve connectivity between the nasal cavity and olfactory bulb
Thallium transport and the evaluation of olfactory nerve connectivity between the nasal cavity and olfactory bulb
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DOI：
10.1093/chemse/bjm066
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发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Miwa, Takaki
中科院分区：
文献类型：
--
作者：
Kinoshita, Yayoi;Shiga, Hideaki;Miwa, Takaki
关于碱金属离子如何在鼻内给药后在嗅神经中转运知之甚少。在这项研究中，我们表明，碱金属离子，铊是在小鼠嗅神经纤维的嗅球。在麻醉状态下，将小鼠的嗅神经纤维在身体两侧横切。第二天将双示踪剂溶液（铊-201，Tl-201;锰-54，Mn-54）注入鼻腔。用伽马能谱法分析嗅球和鼻甲中的放射性。从施用Tl-201或Mn-54的小鼠的冷冻头部的冠状切片获得自动射线照相图像。用神经元示踪剂证实嗅神经纤维的横断。鼻内给药的Tl-201/Mn-54到嗅球的运输被嗅神经纤维的横断显著减少。嗅神经切断也能明显抑制嗅球内氟红宝石的蓄积。研究结果表明，铊是通过嗅觉神经纤维运输到嗅球在小鼠。头部损伤后铊转运的评估可能为嗅觉神经损伤的评估提供一种新的诊断方法。
Little is known regarding how alkali metal ions are transported in the olfactory nerve following their intranasal administration. In this study, we show that an alkali metal ion, thallium is transported in the olfactory nerve fibers to the olfactory bulb in mice. The olfactory nerve fibers of mice were transected on both sides of the body under anesthesia. A double tracer solution (thallium-201, Tl-201; manganese-54, Mn-54) was administered into the nasal cavity the following day. Radioactivity in the olfactory bulb and nasal turbinate was analyzed with gamma spectrometry. Auto radiographic images were obtained from coronal slices of frozen heads of mice administered with Tl-201 or Mn-54. The transection of the olfactory nerve fibers was confirmed with a neuronal tracer. The transport of intranasal administered Tl-201/Mn-54 to the olfactory bulb was significantly reduced by the transection of olfactory nerve fibers. The olfactory nerve transection also significantly inhibited the accumulation of fluoro-ruby in the olfactory bulb. Findings indicate that thallium is transported by the olfactory nerve fibers to the olfactory bulb in mice. The assessment of thallium transport following head injury may provide a new diagnostic method for the evaluation of olfactory nerve injury.