CLONAL ANALYSIS OF HUMAN COLORECTAL TUMORS
CLONAL ANALYSIS OF HUMAN COLORECTAL TUMORS
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DOI：
10.1126/science.2889267
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发表时间：
1987-10-09
期刊：
影响因子：
56.9
通讯作者：
VOGELSTEIN, B
中科院分区：
文献类型：
--
作者：
FEARON, ER;HAMILTON, SR;VOGELSTEIN, B
应用限制性片段长度多态性（RFLPs）技术研究了人结直肠肿瘤的克隆组成。首先，X连锁RFLP被用来检查X染色体失活的模式在女性结直肠肿瘤。所有50个肿瘤检查显示X染色体失活的单克隆模式，这些肿瘤包括20个癌以及30个腺瘤的家族性或自发性类型。第二，利用常染色体的RLSPs作为克隆标记，检测结直肠肿瘤中特定染色体序列的体细胞丢失或获得。在其他变化中，发现染色体17 p序列的体细胞丢失发生在超过75%的癌症中，但这种丢失在腺瘤中很少见。这些数据支持结直肠肿瘤的单克隆起源，并表明17号染色体短臂上的基因可能与从良性到恶性状态的进展有关。
The clonal composition of human colorectal tumors was studied by means of restriction fragment length polymorphisms (RFLPs). First, X-linked RFLPs were used to examine the pattern of X chromosome inactivation in colorectal tumors of females. All 50 tumors examined showed monoclonal patterns of X chromosome inactivation; these tumors included 20 carcinomas as well as 30 adenomas of either familial or spontaneous type. Second, RLSPs of autosomes were used as clonal-markers to detect the somatic-loss or gain of specific chromosomal sequences in colorectal tumors. Among other changes, it was found that somatic loss of chromosome 17p sequences occurred in over 75 percent of the carcinomas examined, but such loss was rare in adenomas. These data support a monoclonal origin for colorectal neoplasms, and suggest that a gene on the short arm of chromosome 17 may be associated with progression from the benign to the malignant state.