Determination of sugar phosphates by high-performance anion-exchange chromatography coupled with pulsed amperometric detection
Determination of sugar phosphates by high-performance anion-exchange chromatography coupled with pulsed amperometric detection
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DOI：
10.1016/j.chroma.2007.07.007
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发表时间：
2007-09-14
影响因子：
4.1
通讯作者：
Hong, Seon-Pyo
中科院分区：
文献类型：
--
作者：
Jeong, Ji-Seon;Kwon, Ha-Jeong;Hong, Seon-Pyo
We have developed an improved analytical method for the determination of sugar phosphates using sodium carbonate (Na2CO3) for high-performance anion-exchange chromatography-pulsed amperometric detection. The target analytes were separated completely within 10 min using eluent containing 20 mM NaOH and 35 mM Na2CO3. The limit of detection (S/N = 3) and quantitation (S/N = 10) for analytes were 10-30 ng/mL and 35-100 ng/mL, respectively. Linear dynamic range was 1-30 mu g/mL (r(2) >= 0.9998). The RSDs for intra- and inter-day assays were found to be of satisfactory results (0.23-3.09%), and the recoveries from blood spots were 97.62-99.69%. (c) 2007 Elsevier B.V. All rights reserved.
