QUANTITATIVE-ANALYSIS OF CONTRACTION BAND AND COAGULATION NECROSIS AFTER ISCHEMIA AND REPERFUSION IN THE PORCINE HEART
QUANTITATIVE-ANALYSIS OF CONTRACTION BAND AND COAGULATION NECROSIS AFTER ISCHEMIA AND REPERFUSION IN THE PORCINE HEART
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DOI：
10.1161/01.cir.75.5.1074
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发表时间：
1987-05-01
期刊：
影响因子：
37.8
通讯作者：
HAMASHIMA, Y
中科院分区：
文献类型：
--
作者：
MIYAZAKI, S;FUJIWARA, H;HAMASHIMA, Y
为评价收缩带坏死（CBN）在再灌注中的重要性，对25只无侧支循环猪心的CBN、凝固性坏死（CN）和梗死面积（CBN + CN）进行了定量分析。结扎冠状动脉左前降支20，30，60，120分钟，然后再灌注8小时（组1至4，分别）。5个心脏没有再灌注（组5）。CBN和CN的面积在放大倍数为100倍下描记。在倒置显微镜下观察100，并使用图像分析仪定量。无论是闭塞还是再灌注，血流动力学均无变化。局部心肌血流量，所产生的氢气清除法测量，减少到几乎为零闭塞后，但在再灌注期间恢复。第1至4组中梗塞的危险区百分比为0 ± 0。0%，11 . ±. 7%，80 . ±. 9%和96 . ±-.第4组中梗死危险区的百分比与永久性闭塞心脏中的相同（96 ± 2%）。3%）。CBN的面积百分比为100 ± 100。第2组为0%，68 . ±.第3组11%，2 . ±.第4组为1%，第4组为2 ± 1%。第五组为2%。第3组缺血左室壁内1/3为CN，中、外1/3为CBN。这些研究结果表明，在猪的心脏没有侧支循环，透壁梗死，其中三分之二被CBN占据，是明显的，即使在1小时闭塞后实现再灌注。因此，对CBN的保护可能会减少再灌注后的梗死面积。
To assess the importance of contraction band necrosis (CBN) in reperfusion, CBN, coagulation necrosis (CN), and infarct size, expressed as CBN + CN, were quantitatively analyzed in 25 porcine hearts without collateral circulation. The left anterior descending coronary artery was ligated for 20, 30, 60, and 120 min and then reperfused for 8 hr (groups 1 to 4, respectively). Five hearts were not reperfused (group 5). The areas of CBN and CN were traced at a magnification of .times. 100 under an inverted microscope and quantified with use of an image analyzer. There was no change in hemodynamics with either occlusion or reperfusion. Regional myocardial blood flow, measured by the generated hydrogen gas clearance method, decreased to almost zero after occlusion but recovered during reperfusion. Percent of risk area infarcted in groups 1 to 4 was 0 .+-. 0%, 11 .+-. 7%, 80 .+-. 9%, and 96 .+-. 2%, respectively, and the percent of risk area infarcted in group 4 was the same as that in hearts subjected to permanent occlusion (96 .+-. 3%). The percent area of CBN was 100 .+-. 0% in group 2, 68 .+-. 11% in group 3, 2 .+-. 1% in group 4, and 2 .+-. 2% in group 5. In group 3, the inner third of the ischemic left ventricular wall showed CN and the middle and outer third CBN. These findings show that in pig hearts without collateral circulation, the transmural infarct, two-thirds of which is occupied by CBN, is evident even when reperfusion is achieved after 1 hr occlusion. Therefore, protection against CBN might reduce infarct size after reperfusion.