Influences of Probe's Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors.
Influences of Probe's Morphology for Metal Ion Detection Based on Light-Addressable Potentiometric Sensors.
复制标题
探针形态对基于光寻址电位传感器金属离子检测的影响
DOI：
10.3390/s16050701
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发表时间：
2016-05-14
期刊：
影响因子：
--
通讯作者：
Jia Y
中科院分区：
文献类型：
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作者：
Shao C;Zhou S;Yin X;Gu Y;Jia Y
将Hg 2+与胸腺嘧啶-胸腺嘧啶（T-T）错配碱基结合的传感机理引入到以抗Hg 2+适体为传感单元的光寻址电位传感器（LAPS）中。设计了3种不同间隔基长度（0 ~ 12 -CH 2）的富T单链DNA（ssDNA），研究了表面电荷和形态对LAPS输出的影响。首先，通过比较三种ssDNA修饰的LAPS对Hg ~（2+）的响应，发现不含-CH ~ 2基团的探针表现出最佳的Hg ~（2+）传感性能。在最佳条件下，Hg ~（2+）离子的检出限为1 ppt。其次，通过控制实验观察了表面电荷和形貌对输出的协同作用。这两种效应分别是金属阳离子平衡的负电荷和T-Hg ~（2+）-T结构的形成引起的形态变化。综上所述，不仅研究了适配体探针的形貌和表面电荷对LAPS的影响，而且首次实现了适配体LAPS对Hg 2+离子的传感。
The sensing mechanism of binding Hg2+ into thymine-thymine (T-T) mismatched base pairs was introduced into a light-addressable potentiometric sensor (LAPS) with anti-Hg2+ aptamer as the sensing units. Three kinds of T-rich single-strand DNA (ssDNA) chains with different spacer lengths, from 0 to 12 –CH2 groups, were designed to investigate surface charge and morphological effects on the LAPS’ output. First, by comparing the responding of LAPS modified with three kinds of ssDNA, it was found that the best performance for Hg2+ sensing was exhibited by the probe without –CH2 groups. The detection limit of Hg2+ ion was 1 ppt under the optimal condition. Second, the cooperative effects of surface charge and morphology on the output were observed by the controlled experiments. The two effects were the negative charge balanced by metal cations and the morphological changing caused by the formation of T-Hg2+-T structure. In conclusion, not only the influences of the aptamer probe’s morphology and surface charge was investigated on the platform of LAPS, but also sensing Hg2+ ions was achieved for the first time by the presented aptamer LAPS.