Simultaneous denitrification and desulfurization-S0 recovery of wastewater in trickling filters by bioaugmentation intervention based on avoiding collapse critical points.
Simultaneous denitrification and desulfurization-S0 recovery of wastewater in trickling filters by bioaugmentation intervention based on avoiding collapse critical points.
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DOI：
10.1016/j.jenvman.2021.112834
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发表时间：
2021-05
影响因子：
8.7
通讯作者：
S. Feng;Zhenming Jiang;Yuqing Chen;Liangqi Gong;Y. Tong;Hailing Zhang;Xing Huang;Hailin Yang
中科院分区：
文献类型：
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作者：
S. Feng;Zhenming Jiang;Yuqing Chen;Liangqi Gong;Y. Tong;Hailing Zhang;Xing Huang;Hailin Yang
为了更好地实现污水的高效同时脱硫脱氮/硫回收，采用硫氧化菌（Sulfur Oxidation Bacteria，简称SBR）和反硝化菌（Sulfurification Bacteria，简称DNB）的干预，以避免负荷波动下的崩溃临界点（S/N去除率急剧下降）。在DNB和NH 4+的辅助作用下，滴滤塔（TF）的坍塌临界点由P8阶段（105-114 d）推迟到P10阶段（129-138 d）。当S/N比为3：1时，氮、硫的处理效率最高。在崩溃临界点添加DNB和NBS可有效调控崩溃状态，使系统最大利用/消除能力分别提高到4.50 kg S m−3·h− 1和0.90 kg N m−3·h−1，比对照提高了56.89%和65.56%。高通量测序分析表明，在反应器的各个阶段，变形菌门（平均78.59%）和拟杆菌门（平均9.30%）均为优势菌。随着反应的进行，微生物群落逐渐被一些具有去除硝酸盐或硫化物功能的菌属所占据，如金黄杆菌属（平均2.97%）、嗜盐硫杆菌属（平均22.71%）、罗丹明杆菌属（平均14.02%）、硫杆菌属（平均9.01%）、硫单胞菌属（平均16.70%）和金属杆菌属（平均21.63%）。主成分分析（PCA）和典型相关分析（CCA）均证实了DNB/DNB在滴滤塔长期运行中的重要作用。
In order to better achieve efficiently simultaneous desulfurization and denitrification/S0recovery of wastewater, the intervention of sulfur oxidizing bacteria (SOB) and denitrifying bacteria (DNB) was employed to avoid the collapse critical points (the dramatically decrease of S/N removal efficiency) under the fluctuated load. With the assistance of DNB and SOB, collapse critical point of trickling filter (TF) was delayed from the P8(105–114 d) to P10stage (129–138 d). The treatment efficiency of nitrogen and sulfur was the highest with the S/N ratio of 3:1. The bioaugmentation of DNB and SOB at collapse critical point could effectively regulated collapse situation, which further increased the maximum system utilization/elimination capacity to 4.50 kg S m−3·h−1and 0.90 kg N m−3·h−1(increased by 56.89% and 65.56% in comparison to control). High-throughput sequencing analysis indicated thatProteobacteria(average 78.59%) andBacteroidetes(average 9.30%) were dominant bacteria in the reactor at all stages. As the reaction proceeds, the microbial community was gradually dominated by some functional genera such asChryseobacterium(average 2.97%),Halothiobacillus(average 22.71%),Rhodanobacter(average 14.02%),Thiobacillus(average 9.01%),Thiomonas(average 16.70%) andMetallibacterium(average 21.63%), which could remove nitrate or sulfide. Both of Principal Component Analysis (PCA) and Canonical Correlation Analysis (CCA) demonstrated the important role of DNB/SOB during the long-term run in the trickling filters (TFs).