Decreased FOXP3 expression in small airways of smokers with COPD
Decreased FOXP3 expression in small airways of smokers with COPD
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DOI：
10.1183/09031936.00145307
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发表时间：
2009-01-01
影响因子：
24.3
通讯作者：
Kratovska, A.
中科院分区：
文献类型：
--
作者：
Isajevs, S.;Taivans, I.;Kratovska, A.
CD 4 + CD 25 + FOXP 3阳性调节性T细胞在控制免疫和炎症反应中具有重要作用。作者推测这些细胞可能参与慢性阻塞性肺疾病（COPD）的发病机制。为探讨FOXP 3在非吸烟者、肺功能正常的吸烟者和COPD患者大、小气道中的表达，选取因孤立性外周型非小细胞肺癌行肺切除术的非吸烟者19例、肺功能正常的吸烟者20例和中度COPD的吸烟者20例。采用免疫组化方法检测肺功能正常的吸烟者和COPD患者的大气道和小气道中FOXP 3的表达，结果显示，与非吸烟者相比，吸烟者和COPD患者的大气道中FOXP 3阳性细胞数量增加。FOXP 3在大气道中的表达与吸烟年龄呈正相关。在小气道中，与无症状吸烟者和非吸烟者相比，COPD患者FOXP 3阳性细胞数量减少，与气流阻塞呈负相关。慢性阻塞性肺病的特征是FOXP 3-的上调在大气道中有阳性细胞，但在与气流受限相关的小气道中下调。本研究结果有助于更好地了解慢性阻塞性肺疾病的发病机制。
CD4+CD25+ FOXP3-positive T-regulatory cells have an important role in controlling immune and inflammatory reactions. The present authors hypothesise that these cells may be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). The aim of the present study was to characterise the expression of FOXP3 in large and small airways of nonsmokers, smokers with normal lung function and COPD patients.A total of 19 nonsmokers, 20 smokers with normal lung function and 20 smokers with moderate COPD, undergoing lung resection for a solitary peripheral nonsmall cell carcinoma, were enrolled in the study. Immunohistochemical methods were used to evaluate FOXP3 expression in large and small airways.Smokers with normal lung function and COPD patients had increased numbers of FOXP3-positive cells in large airways compared with nonsmokers. A positive correlation was observed between FOXP3 expression in large airways and smoked pack-yrs. In small airways, COPD patients had decreased numbers of FOXP3-positive cells, compared with asymptomatic smokers and nonsmokers, that negatively correlated with airflow obstruction.To conclude, chronic obstructive pulmonary disease is characterised by upregulation of FOXP3-positive cells in large airways but a downregulation in small airways that correlated with airflow limitation. The results of the present study contribute to a better understanding of the pathogenesis of chronic obstructive pulmonary disease.