The effect of feeding system in the expression of genes related with fat metabolism in semitendinous muscle in sheep
The effect of feeding system in the expression of genes related with fat metabolism in semitendinous muscle in sheep
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DOI：
10.1016/j.meatsci.2011.04.003
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发表时间：
2011-09-01
期刊：
影响因子：
7.1
通讯作者：
Calvo, J. H.
中科院分区：
文献类型：
--
作者：
Dervishi, E.;Serrano, C.;Calvo, J. H.
研究了饲养系统对半腱肌LPL、ACACA、FASN、FABP 4、DGAT 1、SCD、CPT 1B、PRKAA 2、LEP、SREBP 1、PPARG、PPARA和CEBPB基因表达的影响。选用44只Rasa Aragonesa系单胎雄性羔羊，随机分为放牧苜蓿组、放牧苜蓿+羔羊补饲组、放牧绵羊+室内羔羊组和无水饲养组，结果表明，LPL、ACACA、FABP 4、CPT 1B和SCD基因的表达存在显著差异。与脂肪形成相关的基因（LPL、ACACA、FABP 4和SCD）在强化组中上调。在放牧组中，与VS-氧化过程相关的CPT 1B基因表达上调。CPT 1B在ALF + S组中的相对表达量是对照组的1.54倍，而在IND-GRE和IND组中的相对表达量分别是对照组的0.43和0.37倍，这一结果支持了摄食系统引起的脂肪酸谱变化与脂肪代谢相关基因mRNA表达水平变化的假说。饲养策略是通过改变脂肪代谢相关酶基因表达调控肌肉脂肪酸组成的重要手段。(C)2011爱思唯尔有限公司保留所有权利。
The effect of feeding system on the expression of LPL, ACACA, FASN, FABP4, DGAT1, SCD, CPT1B, PRKAA2, LEP, SREBP1, PPARG, PPARA and CEBPB genes in semitendinous muscle was studied. Forty-four single born male lambs of the Rasa Aragonesa breed, allocated to four different dietary treatments, were used: grazing alfalfa, grazing alfalfa with supplement for lambs, indoor lambs with grazing ewes and drylot.Significant differences were found in the expression of genes LPL, ACACA, FASN, FABP4, CPT1B and SCD. Genes related to adipogenesis (LPL, ACACA, FASN, FABP4, and SCD) are up-regulated in the intensive groups. In grazing groups CPT1B gene expression, related to VS-oxidation process, is up-regulated. The relative expression of CPT1B was 1.54 fold higher in ALF + S, and 0.43 and 0.37 fold lower in IND- GRE and IND, respectively.The results support the hypothesis that changes in fatty acid profile due to feeding system implicate changes in the mRNA expression level of genes related with fat metabolism. Feeding strategy is an important tool to manipulate intramuscular fatty acid profile in meat through altering gene expression of enzymes related with fat metabolism. (C) 2011 Elsevier Ltd. All rights reserved.