Nacre Topography Produces Higher Crystallinity in Bone than Chemically Induced Osteogenesis
Nacre Topography Produces Higher Crystallinity in Bone than Chemically Induced Osteogenesis
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DOI：
10.1021/acsnano.7b01044
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发表时间：
2017-07-01
期刊：
影响因子：
17.1
通讯作者：
Cusack, Maggie
中科院分区：
文献类型：
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作者：
Alakpa, Enateri V.;Burgess, Karl E. V.;Cusack, Maggie
无脊椎动物的贝壳可以诱导骨形成，这是违反直觉的，但珍珠层，或珍珠母，从海洋贝壳是骨诱导和骨整合。珍珠层由文石（碳酸钙）组成，并诱导脊椎动物骨（磷酸钙）的产生。玛雅人将其用于牙科植入物，这种显着的现象已在体外和体内得到证实，但珍珠层诱导骨形成的特征仍然未知。通过分离珍珠层地形从其固有的化学在生产聚己内酯（PCL）珍珠层复制品，我们表明，间充质干细胞，珍珠层地形是骨诱导。与平面PCL相比，骨桥蛋白、骨钙素、骨连接素和osterix的基因表达分别增加了10倍、2倍、1.7倍和1.8倍。此外，委员会还认为，我们证明，响应于珍珠层的物理地形特征而形成的骨组织比响应于化学线索而形成的骨具有更高的结晶度，响应于珍珠层复制品而产生的矿物质的Po-4（3-）拉曼位移的半高宽为7.6 +/- 0.7，而响应于珍珠层复制品而产生的矿物质的半高宽为34.6 +/- 0.7。10.1响应于标准骨诱导介质。矿物质产品的这些差异是由细胞代谢的差异所支撑的。这一观察结果可用于骨治疗的设计;鉴于人口迅速老龄化，这是最紧迫的问题。
It is counterintuitive that invertebrate shells can induce bone formation, yet nacre, or mother of pearl, from marine shells is both osteoinductive and osteointegrative. Nacre is composed of aragonite (calcium carbonate) and induces production of vertebrate bone (calcium phosphate). Exploited by the Mayans for dental implants, this remarkable phenomenon has been confirmed in vitro and in vivo, yet the characteristic of nacre that induces bone formation remains unknown. By isolating nacre topography from its inherent chemistry in the production of polycaprolactone (PCL) nacre replica, we show that, for mesenchymal stem cells, nacre topography is osteoinductive. Gene expression of specific bone marker proteins, osteopontin, osteocalcin, osteonectin, and osterix, is increased 10-, 2-, 1.7-, and 1.8-fold, respectively, when compared to planar PCL. Furthermore, we demonstrate that bone tissue that forms in response to the physical topographical features of nacre has a higher crystallinity than bone formed in response to chemical cues with a full width half-maximum for Po-4(3-) Raman shift of 7.6 +/- 0.7 for mineral produced in response to nacre replica compared to a much broader 34.6 +/- 10.1 in response to standard osteoinductive medium. These differences in mineral product are underpinned by differences in cellular metabolism. This observation can be exploited in the design of bone therapies; a matter that is most pressing in light of a rapidly aging human population.