Long-term inhalation of diesel exhaust affects cytokine expression in murine lung tissues: Comparison between low- and high-dose diesel exhaust exposure
Long-term inhalation of diesel exhaust affects cytokine expression in murine lung tissues: Comparison between low- and high-dose diesel exhaust exposure
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DOI：
10.1080/01902140290096764
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发表时间：
2002-09-01
影响因子：
1.7
通讯作者：
Sugawara, I
中科院分区：
文献类型：
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作者：
Saito, Y;Azuma, A;Sugawara, I
作者研究了柴油机尾气（DE）对海洋肺组织细胞因子表达的影响。BALB/c小鼠暴露于不同剂量水平的DE 1个月（低剂量：柴油机排气颗粒[DEP] 100 mug/m3（3）高剂量：3 mg/m3（3））。暴露后，作者检测了肺中细胞因子（肿瘤坏死因子α [TNF-α]、白细胞介素[IL]-1 β、IL-4、IL-6、IL-10、IL-12 p40和干扰素γ [IFN-γ]）和诱导型一氧化氮合酶（iNOS）的mRNA表达，并测量了肺泡巨噬细胞（AM）分泌TNF-α和IL-10蛋白。炎症细胞因子（TNF-α、IL-1 β、IL-6、IL-12 p40、IFN-γ）和对宿主防御重要的iNOS的mRNA表达水平被显著抑制。DE暴露后IL-10 mRNA水平升高。低剂量DE暴露可使IL-4 mRNA水平升高，而高剂量DE暴露则抑制IL-4 mRNA水平的升高。通过AM β mRNA表达分泌TNF-α和IL-10。慢性吸入DE影响海洋动物肺细胞因子表达。这些结果表明，DE改变肺的免疫反应，并可能增加对病原体的易感性，低剂量DE暴露增加IL-4表达可能诱导过敏反应，如哮喘。
The authors investigated the effect of diesel exhaust (DE) on cytokine expression in marine lung tissues. BALB/c mice were exposed to DE for 1 month at different dose levels of DE (low dose: diesel exhaust particles [DEP] 100 mug/m(3) high dose: 3 mg/m(3)). After exposure, the authors examined mRNA expression of cytokines (tumor nocrosis factor alpha [TFN-alpha], Interleukin [IL]-1beta, IL-4, IL-6, IL-10, IL-12p40, and interferon gamma [IFN-gamma] and inducible nitric oxide synthase (iNOS) in the lung, and also measured the secretion of TNF-alpha and IL-10 protein by alveolar macrophages (AM). The mRNA expression Levels of inflammatory cytokines (TNF-alpha, IL-1beta, IL-6, IL-12p40, IFN-gamma) and iNOS, which are important for host defense, were suppressed significantly. However the IL-10 mRNA level was increased by DE exposure. The IL-4 mRNA level was increased by low-dose DE exposure but suppressed by high-dose DE exposure. TNF-alpha and IL-10 secretion by AM paralleled mRNA expression. Chronic inhalation of DE affects cytokine expression in marine lung. These results suggest that DE alters immunological responses in the lung and may increase susceptibility to pathogens, and that increased IL-4 expression by low-dose DE exposure may induce allergic reaction such as asthma.