Oxysterol sensitive and resistant lymphoid cells: correlation with regulation of cellular nucleic acid binding protein mRNA.
Oxysterol sensitive and resistant lymphoid cells: correlation with regulation of cellular nucleic acid binding protein mRNA.
复制标题
氧甾醇敏感和耐药淋巴细胞：与细胞核酸结合蛋白 mRNA 调节的相关性。
DOI：
10.1016/0960-0760(94)90070-1
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Thompson,EB
中科院分区：
文献类型：
--
作者：
Ayala-Torres,S;Johnson,BH;Thompson,EB
胆固醇的氧化衍生物抑制胆固醇合成，阻止淋巴细胞生长，并引起细胞死亡。我们已经采用了一种新的选择方法，分离M10细胞，一个线的奥施瑞罗耐药细胞，从敏感的克隆CEM C7。占据氧固醇结合蛋白的有效固醇25-羟基胆固醇的浓度在CEM C7中引起细胞死亡，但在M10细胞中不引起。两种细胞系具有相似量的氧固醇结合蛋白，对氧固醇具有相似的亲和力。然而，在这两条线中，氧化固醇结合蛋白mRNA的水平不受1 μM 25-羟基胆固醇的影响。此外，这两种细胞表达细胞核酸结合蛋白（CNBP），7锌指，DNA结合蛋白的未知功能，氧固醇调节。CNBP mRNA的水平显着降低敏感的CEM C7细胞中的25-羟基胆固醇，其中的剂量反应和时间过程与氧固醇的氧固醇结合蛋白的占用率和随后的细胞杀伤一致。然而，在耐药M10细胞中，CNBP mRNA水平不受这些浓度的25-羟基胆固醇的影响。我们的研究结果表明CNBP在氧化甾醇诱导的细胞活力和生长调节中的作用。
Oxygenated derivatives of cholesterol inhibit cholesterol synthesis, prevent lymphoid cell growth, and evoke cell death. We have employed a novel selection method to isolate M10 cells, a line of oxysrerol-resistant cells, from the sensitive clone CEM C7. Concentrations of the potent sterol 25-hydroxycholesterol that occupy the oxysterol binding protein cause cell death in CEM C7, but not in M10 cells. Both cell lines have similar amounts of the oxysterol binding protein with similar affinities for oxysterol. However, in neither line are the levels of oxysterol binding protein mRNA affected by 1 μM 25-hydroxycholesterol. Furthermore, both cells express the cellular nucleic acid binding protein (CNBP), a 7 zinc finger, DNA-binding protein of unknown function, regulated by oxysterols. The levels of CNBP mRNA are significantly reduced by 25-hydroxycholesterol in the sensitive CEM C7 cells, in which the dose response and time course are consistent with occupancy of the oxysterol binding protein by oxysterol and with subsequent cell kill. However, in the resistant M10 cells, CNBP mRNA levels are unaffected by these concentrations of the 25-hydroxycholesterol. Our results suggest a role for CNBP in oxysterol-induced regulation of cell viability and growth.