High-resolution electron cryomicroscopy of macromolecular assemblies
High-resolution electron cryomicroscopy of macromolecular assemblies
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DOI：
10.1016/s0962-8924(99)01511-1
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发表时间：
1999-04-01
影响因子：
19
通讯作者：
Schmid, MF
中科院分区：
文献类型：
--
作者：
Chiu, W;McGough, A;Schmid, MF
电子低温显微镜是一种高分辨率成像技术，特别适用于大分子组装体的结构测定，这些组装体很难通过X射线晶体学或NMR光谱学进行研究。对于某些生物分子，字体二维晶体，电子低温显微镜和图像重建汽车的应用有助于阐明原子分辨率的结构，它的情况下，晶体不能形成，原子分辨率的信息要求通过结合高分辨率的结构，个别组成部分确定的X射线晶体学或核磁共振LR与图像衍生重建在中等分辨率。这可以提供关于这些复合物的机制的独特和关键的信息。最后：图像重建可用于通过提供初始模型来增强X射线研究，所述初始模型促进大分子机器（例如核糖体靶向病毒）晶体定相。
Electron cryomicroscopy is a high-resolution imaging technique that is particularly appropriate for the structural determination of large macromolecular assemblies, which are difficult to study by X-ray crystallography or NMR spectroscopy. For some biological molecules that fonts two-dimensional crystals, the application of electron cryomicroscopy and image reconstruction cars help elucidate structures at atomic resolution, its instances where crystals cannot be formed, atomic-resolution information call be obtained by combining high-resolution structures of individual components determined by X-ray crystallography or NMR LR with image-derived reconstructions at moderate resolution. This can provide unique and crucial information on the mechanisms of these complexes. Finally: image reconstructions can be used to augment X-ray studies by providing initial models that facilitate phasing of crystals of large macromolecular machines such as ribosomes aim viruses.