In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
In vitro regeneration and acclimatization protocols of selected ornamental plants (agapanthus praecox, justicia betonica and celosia cristata)
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发表时间：
2014
期刊：
影响因子：
0.8
通讯作者：
J. S. Yaacob;A. Saleh;H. Elias;S. Abdullah;Noraini Mahmad;Normadiha Mohamed
中科院分区：
文献类型：
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作者：
J. S. Yaacob;A. Saleh;H. Elias;S. Abdullah;Noraini Mahmad;Normadiha Mohamed
以百子莲、白荆和鸡冠花为材料，研究了BAP和NAA生长激素对它们植株再生体系建立的影响。以一个月龄的无菌苗为外植体，研究了不同外植体（叶、茎、茎尖和鳞茎）的生长发育情况。praecox和C. cristata以及来自J.betonica的完整植株的外植体用于实现这些物种的完全植株再生。以MS为基本培养基，添加不同的激素，特别是BAP和NAA，进行直接和间接再生试验。A. praecox（bulbs）和C.鸡冠花在附加0.5-2.0mg/L BAP和NAA的MS培养基上可形成完整植株，而槟榔在附加BAP的MS培养基上可直接再生。采用多种方法对再生菌进行驯化，以A.早熟禾的成活率最高（96.67%），其次是槟榔（80.00%）和水曲柳（80.00%）。嵴形（75.00%）。
This paper discussed on the effectiveness of BAP and NAA growth hormones on establishment of plant regeneration for selected ornamentals; Agapanthus praecox, Justicia betonica and Celosia cristata. Various explants (leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings of A. praecox and C. cristata, as well as explants from intact plants of J. betonica were utilized to achieve complete plant regeneration of these species. MS medium supplemented with various hormones, with an emphasis on BAP and NAA was tested to obtain direct and indirect regeneration. Both A. praecox (bulbs) and C. cristata (shoots) formed complete plantlets on MS added with 0.5-2.0 mg/L BAP and NAA, while direct regeneration was achieved for J. betonica on MS media containing BAP. Several methods were attempted to acclimatize the regenerants, with A. praecox gave the highest percentage of survival rates (96.67%), followed by J. betonica (80.00%) and C. cristata (75.00%).