Selective Zinc(II)‐Ion Fluorescence Sensing by a Functionalized Mesoporous Material Covalently Grafted with a Fluorescent Chromophore and Consequent Biological Applications
Selective Zinc(II)‐Ion Fluorescence Sensing by a Functionalized Mesoporous Material Covalently Grafted with a Fluorescent Chromophore and Consequent Biological Applications
复制标题
DOI：
10.1002/adfm.200800888
复制
发表时间：
2009-01
影响因子：
19
通讯作者：
K. Sarkar;Koushik Dhara;Mahasweta Nandi;P. Roy;A. Bhaumik;P. Banerjee
中科院分区：
文献类型：
--
作者：
K. Sarkar;Koushik Dhara;Mahasweta Nandi;P. Roy;A. Bhaumik;P. Banerjee
用3 -氨基丙基三乙氧基硅烷功能化了一种高度有序的二维六方介孔硅材料。这种有机修饰的介孔材料接枝了一种双醛荧光发色团，4‐甲基‐2,6‐二甲酰苯酚。粉末X射线衍射、透射电子显微镜、N2吸收、傅里叶变换红外光谱、紫外可见吸收和发射等方法对材料进行了表征。该材料表现出优异的选择性Zn2+传感，这是由于其表面存在荧光团部分。荧光测量显示，加入不同浓度的Zn2+后，Zn2+结合的介孔材料的发射强度显著增加，而引入其他生物相关（Ca2+, Mg2+， Na+和K+）和环境有害的过渡金属离子导致强度不变或减弱。荧光增强归因于Zn2+的强共价结合，从大的结合常数值（0.87 × 104 M−1）可以看出。因此，这种接枝荧光发色团的功能化介孔材料可以从含有微量Zn2+的离子混合物中监测或识别Zn2+，可以被认为是一种选择性荧光探针。我们用荧光显微镜研究了这种介孔锌（II）传感器在培养活细胞（A375人黑色素瘤和人宫颈癌细胞，HeLa）中的应用。
A highly ordered 2D‐hexagonal mesoporous silica material is functionalized with 3‐aminopropyltriethoxysilane. This organically modified mesoporous material is grafted with a dialdehyde fluorescent chromophore, 4‐methyl‐2,6‐diformyl phenol. Powder X‐ray diffraction, transmission electron microscopy, N2 sorption, Fourier transform infrared spectroscopy, and UV‐visible absorption and emission have been employed to characterize the material. This material shows excellent selective Zn2+ sensing, which is due to the fluorophore moiety present at its surface. Fluorescence measurements reveal that the emission intensity of the Zn2+‐bound mesoporous material increases significantly upon addition of various concentrations of Zn2+, while the introduction of other biologically relevant (Ca2+, Mg2+, Na+, and K+) and environmentally hazardous transition‐metal ions results in either unchanged or weakened intensity. The enhancement of fluorescence is attributed to the strong covalent binding of Zn2+, evident from the large binding constant value (0.87 × 104 M−1). Thus, this functionalized mesoporous material grafted with the fluorescent chromophore could monitor or recognize Zn2+ from a mixture of ions that contains Zn2+ even in trace amounts and can be considered as a selective fluorescent probe. We have examined the application of this mesoporous zinc(II) sensor to cultured living cells (A375 human melanoma and human cervical cancer cell, HeLa) by fluorescence microscopy.