In vivo and in vitro morphological analysis of melanocytes homozygous for the mi(sp) allele at the murine microphthalmia locus
In vivo and in vitro morphological analysis of melanocytes homozygous for the mi(sp) allele at the murine microphthalmia locus
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DOI：
10.1111/j.1600-0749.1995.tb00677.x
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发表时间：
1995-12-01
期刊：
影响因子：
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通讯作者：
Lamoreux, ML
中科院分区：
文献类型：
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作者：
Boissy, RE;Lamoreux, ML
mi（sp）等位基因（斑点状小眼畸形）是小鼠小眼畸形（mi）基因座的突变等位基因，当纯合子时，导致正常表型，其中皮毛色素沉着或眼发育没有明显改变。然而，当与其他mi基因座等位基因，特别是Mi（uh）（小眼白）杂合子时，mi（.8p）等位基因对表型产生影响。我们检测了C57 BL/6 J-mi（.sp）/mi（. 8 p）小鼠、C57 BL/6 J-Mi（.uh）/Mi（.uk）小鼠、C57 BL/6 J-Mi（.uh）/mi 2（. 8 p）小鼠和C57 BL/6 J-（+）/（+）对照小鼠生长期毛球和脉络膜以及视网膜色素上皮中黑素细胞的超微结构。mi（.8p）/mi（.8p）小鼠的黑素细胞原位表现正常。然而，来自mi（.sp）/mi（.8p）小鼠的新生皮肤的黑素细胞培养物表现出小的原代集落，其大小没有显著扩大。偶尔，在mi（.8p）/mi（.8p）黑素细胞的原代培养物中观察到高尔基体结构的异常。这些结果表明，虽然mi（.8p）等位基因对小鼠的表型没有明显影响，但它确实显著抑制了正常培养条件下黑素细胞的存活。
The mi(sp) allele (microphthalmia-spotted), a mutant allele at the murine microphthalmia (mi) locus, when homozygous, results in a normal phenotype in which there is no apparent alteration in pelage pigmentation or ocular development. However, when heterozygous with other mi locus alleles, specifically Mi(uh) (microphthalmia-white) the mi(.8p) allele exerts an affect on the phenotype. We examined the ultrastructure of melanocytes in the anagen hair bulb and the choroid plus the retinal pigmented epithelium of C57BL/6J-mi(.sp)/mi(.8p) mice, C57BL/6J-Mi(.uh)/Mi(.uk) mice, C57BL/6J-Mi(.uh)/mi2(.8p) mice, and C57BL/6J-(+)/(+) control mice. Melanocytes of the mi(.8p)/mi(.8p) mice appeared normal in situ. However, melanocyte cultures derived from neonatal skins of mi(.sp)/mi(.8p) mice exhibited small primary colonies that did not dramatically expand in size. Occasionally, abnormalities in the structure of the Golgi apparatus were observed in primary cultures of mi(.8p)/mi(.8p) melanocytes. These results demonstrate that while the mi(.8p) allele has no obvious effect on the phenotype of the mouse, it does dramatically suppress the survival of melanocytes in normal culture conditions.