Genetic variability of the common nod gene in soybean bradyrhizobia isolated in Thailand and Japan.
Genetic variability of the common nod gene in soybean bradyrhizobia isolated in Thailand and Japan.
复制标题
泰国和日本分离的大豆慢生根瘤菌普通结基因的遗传变异。
DOI：
10.1139/m96-156
复制
发表时间：
1996
影响因子：
2.8
通讯作者：
H. Imai
中科院分区：
文献类型：
--
作者：
T. Yokoyama;S. Ando;T. Murakami;H. Imai
为确定泰国大豆慢生根瘤菌与其他地区大豆慢生根瘤菌的分类关系，共分离到62株泰国大豆慢生根瘤菌。对46株日本菌株和15株美国农业部菌株的遗传多样性进行了检测。用日本慢生型根瘤菌USDA 110普通结节DYABC基因探针进行限制性片段长度多态性分析，估计123株菌株共同结瘤基因区域及其周围的序列差异程度。这些菌株的系统发育分类产生了四个主要的聚类群。第一组包括日本和美国农业部的毒株，它们起源于温带地区，而第三和第四组包括热带泰国毒株。群1菌株由DNA同源群I和Ia组成，因此被归类为B.Japan onicum。群2菌株属于DNA同源群II，因此被归类为慢生根瘤菌Elkanii。然而，群3和群4的菌株并不对应于任何已知的DNA同源组。这些结果表明，泰国大豆慢生根瘤菌与日本大豆根瘤菌和埃尔坎大豆根瘤菌有较远的亲缘关系。
To determine the taxonomic relationship between Thai soybean bradyrhizobia and soybean bradyrhizobia from other regions, a total of 62 Bradyrhizobium strains were isolated in Thailand. The genetic diversity of the strains was examined with reference to 46 Japanese and 15 USDA strains. The degree of sequence divergence in and around common nod gene regions of the 123 strains was estimated by restriction fragment length polymorphism analysis using the Bradyrhizobium japonicum USDA 110 common nodDYABC gene probe. The phylogenetic grouping of the strains resulted in four major clusters. Cluster 1 comprised the Japanese and USDA strains, which originated in temperate regions, whereas clusters 3 and 4 comprised the tropical Thai strains. Cluster 1 strains comprised the DNA homology groups I and Ia, and hence, were classified as B. japonicum. Cluster 2 strains were in the DNA homology group II, and hence, were classified as Bradyrhizobium elkanii. Clusters 3 and 4 strains, however, did not correspond to any known DNA homology groups. These results indicate that Thai soybean bradyrhizobia are distantly related to B. japonicum and B. elkanii.