Complete nucleotide sequences of Nipah virus isolates from Malaysia
Complete nucleotide sequences of Nipah virus isolates from Malaysia
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DOI：
10.1099/0022-1317-82-9-2151
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发表时间：
2001-09-01
影响因子：
3.8
通讯作者：
Lam, SK
中科院分区：
文献类型：
--
作者：
Chan, YP;Chua, KB;Lam, SK
我们已经对两种尼帕病毒分离株的基因组进行了完全测序，一种来自马来西亚唯一存活的脑脊液病毒分离阳性脑脊液患者的喉咙分泌物，另一种来自脑脊液。这两个基因组各有18246个核苷酸，只相差4个核苷酸。NiV基因组比亨德拉病毒（HeV）基因组长12个核苷酸，并且两个基因组具有相同的前导和后导序列长度以及所有基因的六聚体相位位置。NiV和HeV在基因组末端序列、基因启动和停止信号、P基因编辑信号以及推断出的核衣壳蛋白、磷蛋白、基质蛋白、融合蛋白、糖蛋白和RNA聚合酶的氨基酸序列方面也非常相似。现有证据表明，显然需要在副粘病毒亚科中创建一个新属，以适应NiV和HeV之间的密切相似性以及它们与该亚科其他成员的显著差异。
We have completely sequenced the genomes of two Nipah virus (NiV) isolates, one from the throat secretion and the other from the cerebrospinal fluid (CSF) of the sole surviving encephalitic patient with positive CSF virus isolation in Malaysia. The two genomes have 18246 nucleotides each and differ by only 4 nucleotides. The NiV genome is 12 nucleotides longer than the Hendra virus (HeV) genome and both genomes have identical leader and trailer sequence lengths and hexamer-phasing positions for all their genes. Both NiV and HeV are also very closely related with respect to their genomic end sequences, gene start and stop signals, P gene-editing signals and deduced amino acid sequences of nucleocapsid protein, phosphoprotein, matrix protein, fusion protein, glycoprotein and RNA polymerase. The existing evidence demonstrates a clear need for the creation of a new genus within the subfamily Paramyxovirinae to accommodate the close similarities between NiV and HeV and their significant differences from other members of the subfamily.