Gene-Gene Interactions Between Interleukin-12A and Interleukin-12B with the Risk of Brain Tumor
Gene-Gene Interactions Between Interleukin-12A and Interleukin-12B with the Risk of Brain Tumor
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Interleukin-12A 和 Interleukin-12B 之间的基因间相互作用与脑肿瘤风险
DOI：
10.1089/dna.2011.1331
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发表时间：
2012-02-01
影响因子：
3.1
通讯作者：
You, Chao
中科院分区：
文献类型：
--
作者：
Sima, Xiutian;Xu, Jianguo;You, Chao
来自临床前和临床研究的新证据表明，白细胞介素-12（IL-12）对内源性脑肿瘤具有一定的疗效。本研究的目的是探讨IL-12 A和IL-12 B多态性在脑肿瘤风险中的相互作用。我们采用聚合酶链反应-限制性片段长度多态性分析和DNA测序方法分析了170例脑肿瘤患者和222例健康对照者的IL-12 A rs 2243115和IL-12 B rs3212227多态性。携带IL-12 A rs 2243115的G等位基因的个体与携带T等位基因的个体相比具有显著更高的发展脑肿瘤的风险（比值比[OR] = 2.01，95%置信区间[CI]，1.17-3.45）。根据肿瘤类型分层分析后，在胶质瘤患者中检测到类似的较高风险（OR = 2.56，95%CI，1.25-5.21）。当检测基因-基因交互作用时，同时携带rs 2243115 TG/GG和rs3212227 AC/CC基因型的患者发生胶质瘤的风险是携带rs 2243115 TT和rs3212227 AC/CC基因型患者的2.62倍（OR = 2.62，95%CI，1.05-6.50）。这项研究提供的证据表明，IL-12 A rs 2243115可能与脑肿瘤的风险有关。此外，IL-12 A rs 2243115和IL-12 B rs3212227的基因基因相互作用可能有助于脑肿瘤易感性。
Emerging evidence from preclinical and clinical studies has shown that interleukin-12 (IL-12) has some effectiveness against endogenously arising brain tumor. The aim of this study was to investigate interactions of IL-12A and IL-12B polymorphisms on the risk of brain tumor. We analyzed IL-12A rs2243115 and IL-12B rs3212227 polymorphisms in 170 patients with brain tumor and 222 healthy controls in a Chinese population using a polymerase chain reaction-restriction fragment length polymorphism assay and DNA sequencing method. Individuals carrying a G allele of IL-12A rs2243115 had a significantly higher risk of developing brain tumor compared with those carrying a T allele (odds ratio [OR] = 2.01, 95% confidence interval [CI], 1.17-3.45). After stratification analysis according to tumor types, a similarly higher risk was detected in patients with glioma (OR = 2.56, 95% CI, 1.25-5.21). When gene gene interactions were examined, carriers at both loci rs2243115 TG/GG and rs3212227 AC/CC had a 2.62-fold increased risk of glioma compared with those with rs2243115 TT and rs3212227 AC/CC genotypes (OR = 2.62, 95% CI, 1.05-6.50). This study provides evidence that IL-12A rs2243115 may be associated with the risk of brain tumor. Additionally, gene gene interactions of IL-12A rs2243115 and IL-12B rs3212227 may contribute to brain tumor susceptibility.