Whole-genome sequencing reveals that mutations in myosin-5 confer resistance to the fungicide phenamacril in Fusarium graminearum.
Whole-genome sequencing reveals that mutations in myosin-5 confer resistance to the fungicide phenamacril in Fusarium graminearum.
复制标题
全基因组测序表明，myosin-5 的突变导致禾谷镰刀菌对杀菌剂 phenamacril 产生抗性。
DOI：
10.1038/srep08248
复制
发表时间：
2015-02-04
影响因子：
4.6
通讯作者：
Zhou M
中科院分区：
文献类型：
--
作者：
Zheng Z;Hou Y;Cai Y;Zhang Y;Li Y;Zhou M
为了明确禾谷镰刀菌（Fusarium graminearum）对杀菌剂苯那普利（phenamacril，JS 399 -19）的抗性机制，我们对禾谷镰刀菌（Fusarium graminearum）的抗性菌株YP-1进行了基因组测序和注释。禾谷镰刀菌参考菌株PH-1与苯那普利）。在基于Illumina的配对末端测序测定的总共140万个读数中，92.80%与F.禾本科参考基因组。与菌株PH-1相比，菌株YP-1含有1，989个单核苷酸多态性，导致132个基因的氨基酸突变。对另一株F.禾谷镰刀菌敏感菌株2021和相应的抗性菌株。所有耐药突变体的唯一共同突变发生在编码肌球蛋白-5的基因中（密码子216、217、418、420或786处的点突变）。为了确认肌球蛋白-5的突变是否赋予对非那普利的抗性，我们通过同源双交换在敏感菌株2021和抗性菌株Y2021 A之间交换了肌球蛋白-5位点。具有抗性片段拷贝的转化突变体表现出对非那普利的抗性，而具有敏感片段拷贝的转化突变体表现出对非那普利的敏感性。这些结果表明，肌球蛋白-5的突变赋予耐药性的非那普利在F。禾谷早熟禾
To determine the mechanism of resistance to the fungicide phenamacril (JS399-19) in Fusarium graminearum, the causal agent of Fusarium head blight, we sequenced and annotated the genome of the resistant strain YP-1 (generated by treating the F. graminearum reference strain PH-1 with phenamacril). Of 1.4 million total reads from an Illumina-based paired-end sequencing assay, 92.80% were aligned to the F. graminearum reference genome. Compared with strain PH-1, strain YP-1 contained 1,989 single-nucleotide polymorphisms that led to amino acid mutations in 132 genes. We sequenced 22 functional annotated genes of another F. graminearum sensitive strain (strain 2021) and corresponding resistant strains. The only mutation common to all of the resistant mutants occurred in the gene encoding myosin-5 (point mutations at codon 216, 217, 418, 420, or 786). To confirm whether the mutations in myosin-5 confer resistance to phenamacril, we exchanged the myosin-5 locus between the sensitive strain 2021 and the resistant strain Y2021A by homologous double exchange. The transformed mutants with a copy of the resistant fragment exhibited resistance to phenamacril, and the transformed mutant with a copy of the sensitive fragment exhibited sensitivity to phenamacril. These results indicate that mutations in myosin-5 confers resistance to phenamacril in F. graminearum.