CHARACTERIZATION OF A HUMAN CLASS-THETA GLUTATHIONE-S-TRANSFERASE WITH ACTIVITY TOWARDS 1-MENAPHTHYL SULFATE
CHARACTERIZATION OF A HUMAN CLASS-THETA GLUTATHIONE-S-TRANSFERASE WITH ACTIVITY TOWARDS 1-MENAPHTHYL SULFATE
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DOI：
10.1042/bj2860929
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发表时间：
1992-09-15
影响因子：
4.1
通讯作者：
HAYES, JD
中科院分区：
文献类型：
--
作者：
HUSSEY, AJ;HAYES, JD
本文介绍了一种从人肝中纯化谷胱甘肽S-转移酶（GST）的方法，该酶催化1-甲基萘硫酸酯（MS）与GSH的结合，所设计的方法可得到约100%的GST。500-代谢MS的人酶是包含M（r）25 - 100的亚基的同二聚体，并且免疫化学实验已经表明它是Theta类GST的成员。该酶的自动Edman降解已证实它是θ-类GST，但获得的氨基酸序列不同于先前所述的GST-θ的氨基酸序列[Meyer，Coles，Pemble，Gilmore，Fraser & Ketterer（1991）Biochem.J.274，409 -414]。因此，我们指定了催化MS与GSH GST T2-2* 结合的酶（在缺乏完整氨基酸序列数据的情况下，T1和T2亚基暂时称为T1* 和T2*）;讨论了表明GST-θ（现在可能称为GST T1-1*）和GST T2-2* 代表不同同工酶的证据。
A purification scheme is described for a glutathione S-transferase (GST) from human liver that catalyses the conjugation of 1-menaphthyl sulphate (MS) with GSH, the method devised results in an approx. 500-fold increase in specific activity towards MS. The human enzyme which metabolizes MS is a homodimer comprising subunits of M(r) 25 100, and immunochemical experiments have shown it to be a member of the class-Theta GSTs. Automated Edman degradation of this enzyme has confirmed that it is a Theta-class GST but the amino acid sequence obtained differs from that of GST-theta described previously [Meyer, Coles, Pemble, Gilmore, Fraser & Ketterer (1991) Biochem. J. 274,409-414]. We have therefore designated the enzyme that catalyses the conjugation of MS with GSH GST T2-2* (in the absence of complete amino acid sequence data, the T1 and T2 subunits are provisionally designated T1* and T2*); the evidence which indicates that GST-theta (which should possibly now be called GST T1-1*) and GST T2-2* represent distinct isoenzymes is discussed.