Single cell membrane poration by bubble-induced microjets in a microfluidic chip
Single cell membrane poration by bubble-induced microjets in a microfluidic chip
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DOI：
10.1039/c3lc41252k
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Ohl, C. D.
中科院分区：
文献类型：
--
作者：
Li, Z. G.;Liu, A. Q.;Ohl, C. D.
本文论证了快速液体微射流对单个悬浮池的膜穿孔。该喷射是在激光诱导的气泡破裂期间形成的，该气泡在与目标细胞的可变距离处产生。该细胞被微流控芯片内的聚合结构捕获。细胞附近空化气泡的不对称生长和坍塌导致微射流，使细胞膜变形和穿孔。在实验中，用台盼蓝摄取来探测骨髓瘤细胞的膜部分。台盼蓝扩散的时间分辨研究表明，它与气泡动力学有明显的依赖关系，即距离。染料的穿透长度随距离的缩短而增大。扩散过程的数值模拟与细胞膜上形成的更大的孔一致。这种方法允许快速、可重复和局部地破裂悬浮液中的单个细胞膜。
This paper demonstrates membrane poration of a single suspension cell due to a fast liquid microjet. The jet is formed during the collapse of a laser induced bubble created at a variable stand-off distance from the target cell. The cell is trapped by a converging structure within a microfluidic chip. The asymmetrical growth and collapse of the cavitation bubble next to the cell lead to the microjetting, which deforms and porates the cell membrane. In the experiments, the membrane porations of myeloma cells are probed with the uptake of trypan blue. Time-resolved studies of the diffusion of trypan blue show a marked dependency on the bubble dynamics, i.e. the stand-off distance. The penetration length of the dye increases with shorter distances. Numerical simulations of the diffusion process agree with larger pores formed on the cell membrane. This method allows for a fast, repeatable, and localized rupture of membranes of individual cells in suspension.