MIGRATION AND HOMING OF LYMPHOCYTES TO LYMPHOID AND SYNOVIAL TISSUES IN PROTEOGLYCAN-INDUCED MURINE ARTHRITIS
MIGRATION AND HOMING OF LYMPHOCYTES TO LYMPHOID AND SYNOVIAL TISSUES IN PROTEOGLYCAN-INDUCED MURINE ARTHRITIS
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DOI：
10.1002/art.1780370919
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发表时间：
1994-09-01
影响因子：
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通讯作者：
GLANT, TT
中科院分区：
文献类型：
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作者：
MIKECZ, K;GLANT, TT
Objective.描述在软骨蛋白聚糖（PG）诱导的关节炎的发展过程中，标记的供体淋巴细胞向宿主动物的淋巴器官和滑膜组织的迁移和归巢。来自非关节炎或关节炎供体动物的淋巴细胞用荧光或放射性细胞连接物（PKH-GL）标记，并注射到同基因的免疫抑制小鼠中。通过荧光显微镜和组织样品放射性测定研究了标记细胞注射后供体淋巴细胞的归巢模式。来自关节炎供体的淋巴细胞在标记后保留了转移疾病的能力。在淋巴器官中，关节炎和nonarthritic捐助者淋巴细胞表现出类似的归巢模式，虽然显着的差异，发现归巢细胞的数量来自nonarthritic和关节炎捐助者。然而，标记细胞仅从关节炎动物迁移到受体小鼠的滑膜组织中，并且它们的出现与关节炎的发生有关。与非关节炎供体的淋巴细胞相比，PG诱导的关节炎小鼠的淋巴细胞表现出优先归巢宿主的滑膜组织。关节炎的恶性转移与滑膜中这些细胞的出现有关。
Objective. To describe the migration and homing of labeled donor lymphocytes to the lymphoid organs and synovial tissues of host animals, during the development of cartilage proteoglycan (PG)-induced arthritis adoptively transferred to syngeneic BALB/c mice.Methods. Lymphocytes from either nonarthritic or arthritic donor animals were labeled with either fluorescent or radioactive cell linkers (PKH-GL) and injected into syngeneic, immunosuppressed mice. The homing patterns of donor lymphocytes following the injection of labeled cells were studied by fluorescence microscopy and by measurement of radioactivity in tissue samples.Results. Lymphocytes from arthritic donors retained their ability to transfer the disease after labeling. In the lymphoid organs, arthritic and nonarthritic donor lymphocytes exhibited similar homing patterns, although remarkable differences were found in the number of homing cells derived from nonarthritic and arthritic donors. However, labeled cells only from arthritic animals migrated to the synovial tissue of the recipient mice, and their appearance was associated with the onset of arthritis.Conclusion. Lymphocytes from mice with PG-induced arthritis, in contrast to lymphocytes from nonarthritic donors, exhibit preferential homing to the synovial tissue of the host. Adoptive transfer of arthritis is linked to the appearance of these cells in the synovium.