CHROMOSOME-BANDING HOMOLOGIES IN 3 SPECIES OF AEDES (STEGOMYIA)
CHROMOSOME-BANDING HOMOLOGIES IN 3 SPECIES OF AEDES (STEGOMYIA)
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DOI：
10.1139/g86-027
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发表时间：
1986-04-01
期刊：
影响因子：
--
通讯作者：
RAI, KS
中科院分区：
文献类型：
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作者：
MARCHI, A;RAI, KS
属于隐蚊亚属的埃及伊蚊、马斯卡伊蚊和白纹伊蚊的染色体互补体对 Q、H 和 R 显带技术给出了一致的响应。在三个同态染色体对中，只有最短的或性别对 (I) 显示出一致的带型。在这三个物种中，经过热处理并用吖啶橙染色后，两性染色体的一条臂上都出现了一条亮黄色的嵌间带。染色体的其余部分和另外两对发出橙红色荧光。相同的居间区域在荧光染料奎纳克林和 Hoechst 33258 的作用下显得完全黑暗，而染色体的其余部分发出暗淡的荧光。男性和女性中存在相同的条带图案。在每个物种内观察到 Q- 和 H- 阴性以及 R- 阳性闰带的大小变化。结果根据性别决定染色体的结构同源性进行解释，该染色体保留在亚属内。
The chromosome complements of the mosquitoes Aedes aegypti, Aedes mascarensis, and Aedes albopictus, belonging to the subgenus Stegomyia, gave a uniform response to the Q-, H-, and R-banding techniques. Of the three homomorphic chromosome pairs, only the shortest or sex pair (I) showed a consistent banding pattern. In the three species, a bright yellow intercalarly band was present on one arm of both chromosomes of the sex pair after heat treatment and staining with acridine orange. The rest of the chromosome and the other two pairs fluoresced orange-red. The same intercalary region appeared completely dark with the fluorochromes quinacrine and Hoechst 33258, while the rest of the chromosomes fluoresced dull. The same banding pattern was present in males and females. Size variations of the Q- and H-negative and R-positive intercalary bands were observed within each species. The results are interpreted in terms of structural homology of the sex-determining chromosomes, which is retained within the subgenus.