Inhibitory Action of Chlorpromazine on the Oxidation of D-Amino-Acid in the Diencephalon Part of the Brain
Inhibitory Action of Chlorpromazine on the Oxidation of D-Amino-Acid in the Diencephalon Part of the Brain
复制标题
氯丙嗪对间脑D-氨基酸氧化的抑制作用
作者：
K. Yagi;T. Nagatsu;T. Ozawa
在本实验室研究的几种影响D-氨基酸氧化酶活性的物质中，发现氯丙嗪（N-（3′-二甲氨基丙基）-3-氯吩噻嗪盐酸盐）是该酶的强抑制剂。用纯化的氧化酶系统和瓦尔堡压力计研究了其抑制机理，并进行了动力学研究。采用Negelein和Brömel法从猪肾中提取D-氨基酸氧化酶蛋白。每种酶系统由脱辅基酶（蛋白E）、1.6 × 10−7摩尔的由八木等人2的方法制备的黄素腺嘌呤二核苷酸、6.25 × 10−2摩尔的D-丙氨酸组成。向这些酶体系中加入2 × 10 - 5至4 × 10 - 6摩尔的氯丙嗪（由Rhône-Poulenc-Specia提供）。
AMONG several substances examined in our laboratory for their influence on the activity of D-amino-acid oxidase, chlorpromazine (N-(3′-dimethylaminopropyl)-3-chlorophenothiazine hydrochloride) was found to be a powerful inhibitor of this enzyme. The inhibitory mechanism was investigated using a purified oxidase enzyme system and Warburg manometer, and the kinetic study carried out. D-Amino-acid oxidase protein was prepared from hog kidney by the method of Negelein and Brömel1. Each enzyme system consisted of apo-enzyme (protein E), 1.6 × 10−7 mole of flavin adenine dinucleotide prepared by the method of Yagi et al.2, 6.25 × 10−2 mole of D-alanine. Amounts of chlorpromazine (supplied by Rhône-Poulenc-Specia) ranging from 2 × 10−5 to 4 × 10−6 mole were added to these enzyme systems.