Microwave-assisted rapid synthesis of luminescent gold nanoclusters for sensing Hg2+ in living cells using fluorescence imaging
Microwave-assisted rapid synthesis of luminescent gold nanoclusters for sensing Hg2+ in living cells using fluorescence imaging
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DOI：
10.1039/c2nr30219e
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发表时间：
2012-01-01
期刊：
影响因子：
6.7
通讯作者：
Nienhaus, G. Ulrich
中科院分区：
文献类型：
--
作者：
Shang, Li;Yang, Linxiao;Nienhaus, G. Ulrich
研究了一种微波辅助合成二氢硫辛酸（DHLA）覆盖荧光金纳米团簇（aunc）的方法。在合成过程中，微波照射使aunc的荧光量子产率提高了约5倍，并将反应时间从几小时缩短到几分钟。所合成的DHLA-AuNCs具有明亮的近红外荧光（QY: 2.9%），超小的水动力直径（3.3 nm），在生理相关的pH值5-10范围内具有良好的胶体稳定性，并且对HeLa细胞具有低的细胞毒性。此外，这些dhla - aunc能够通过Hg2+和Au+在aunc表面的特异性相互作用来感知Hg2+；检测限（LOD）为0.5 nM。旋盘共聚焦显微镜在HeLa细胞内Hg2+成像中的潜在应用。
A microwave-assisted strategy for synthesizing dihydrolipoic acid (DHLA) capped fluorescent gold nanoclusters (AuNCs) has been developed. Irradiation with microwaves during synthesis enhanced the fluorescence quantum yield (QY) of AuNCs by about five-fold and shortened the reaction time from hours to several minutes. The as-synthesized DHLA-AuNCs possessed bright near-infrared fluorescence (QY: 2.9%), ultrasmall hydrodynamic diameter (3.3 nm), good colloidal stability over the physiologically relevant pH range of 5-10 as well as low cytotoxicity toward HeLa cells. Moreover, these DHLA-AuNCs were capable of sensing Hg2+ through the specific interaction between Hg2+ and Au+ on the surface of AuNCs; the limit of detection (LOD) was 0.5 nM. A potential application in imaging intracellular Hg2+ in HeLa cells was demonstrated by using spinning disc confocal microscopy.