Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues
Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues
复制标题
开发异源酶联免疫吸附测定法，用于检测可食用动物组织中的克林霉素和林可霉素残留。
DOI：
10.1016/j.meatsci.2016.11.024
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发表时间：
2017-03-01
期刊：
影响因子：
7.1
通讯作者：
Jiang, Wenxiao
中科院分区：
文献类型：
--
作者：
He, Jie;Wu, Nan;Jiang, Wenxiao
本研究制备了新型克林霉素(CLIN)人工抗原，并将其用于制备广特异性的单抗。以制备的单抗为基础，建立了检测可食用动物组织中CLIN和林可霉素(LIN)残留的异源ELISA法。在缓冲液中的IC50值分别为0.3 ng/m L(CLIN)和1.2 ng/m L(LIN)。牛、鸡、猪和鱼肉的检出限分别为1.8mg/kg(CLIN)和6.8mg/kg(LIN)。在加标和回收试验中，不同加标水平的平均回收率为76%~112%，批内/批间变异系数为7.1%~13.2%。用LC-MS/MS进行了验证，相关系数为>；0.97。该方法适用于牛、鸡、猪、鱼等肌肉中CLIN和LIN残留量的同时检测。(C)2016爱思唯尔有限公司。保留所有权利。
In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC50 values of the developed assay were 0.3 ng/mL (CLIN) and 1.2 ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8 mu g/kg (CLIN) and 6.8 mu g/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra -/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC - MS/MS with a correlation coefficient >0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles. (C) 2016 Elsevier Ltd. All rights reserved.