ANALYSIS OF WATER-MACROMOLECULE PROTON MAGNETIZATION TRANSFER IN ARTICULAR-CARTILAGE
ANALYSIS OF WATER-MACROMOLECULE PROTON MAGNETIZATION TRANSFER IN ARTICULAR-CARTILAGE
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DOI：
10.1002/mrm.1910290209
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发表时间：
1993-02-01
影响因子：
3.3
通讯作者：
BALABAN, RS
中科院分区：
文献类型：
--
作者：
KIM, DK;CECKLER, TL;BALABAN, RS
这些研究旨在确定软骨的哪些结构元素负责在关节软骨的MRI研究中观察到的水（Hf）和大分子（Hr）之间的质子磁化转移。饱和转移技术用于监测软骨的两种主要成分：胶原蛋白和蛋白多糖样品的体外磁化转移。在去除蛋白多糖部分之前和之后，关节软骨样品也在体外进行了评估。分离的水合胶原蛋白在水中表现出显著的质子磁化转移率。相比之下，蛋白聚糖没有表现出质子磁化转移。关节软骨在体外表现出高度的水质子磁化转移，这与之前在体内的MRI研究一致。从软骨中去除蛋白多糖并不会改变Hr和Hf之间的磁化转移速率。这些数据表明，胶原基质的结构和浓度是关节软骨磁化转移过程的主要决定因素，而蛋白聚糖的贡献很少或没有贡献。这种磁化转移效应的特异性可能在体内软骨组成和结构的无创评估中被证明是有用的。
These studies were designed to establish which structural elements of cartilage are responsible for proton magnetization transfer between water (Hf) and macromolecules (Hr) observed in MRI studies on articular cartilage. Saturation transfer techniques were used to monitor magnetization transfer in vitro on samples of the two major constituents of cartilage: collagen and proteoglycan. Articular cartilage samples were also evaluated in vitro before and after the removal of the proteoglycan fraction. Isolated hydrated collagen exhibited a significant proton magnetization transfer rate with water. In contrast, proteoglycans exhibited no proton magnetization transfer. Articular cartilage, in vitro, exhibited a high degree of magnetization transfer with water protons consistent with previous MRI studies in vivo. Enzymatic removal of proteoglycan from the cartilage did not alter the magnetization transfer rate between Hr and Hf. These data demonstrate that the structure and concentration of the collagen matrix are the predominant determinants of the magnetization transfer process in articular cartilage with little or no contribution from proteoglycans. This specificity of the magnetization transfer effect may prove useful in the noninvasive evaluation of cartilage composition and structure in vivo.