Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells.
Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells.
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DOI：
10.3892/ijo.30.5.1231
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发表时间：
2007-05
影响因子：
5.2
通讯作者：
Jeffery G Herman;G. Meadows
中科院分区：
文献类型：
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作者：
Jeffery G Herman;G. Meadows
3类信号蛋白sema3A和sema3C在细胞发育和癌症中提供重要的指导信号；然而，这些信号蛋白在前列腺癌中的作用尚不清楚。我们在此报道，在基于matrigel的检测中，转染sema3A的细胞表现出侵袭性和粘附性降低，而转染sema3C的细胞表现出侵袭性和粘附性增强。重要的粘附蛋白在sema3A和sema3C细胞中以与其随后的侵袭和粘附特性一致的方式进行差异调节。Western blot结果显示，与未转染和模拟空载体转染的PC-3细胞相比，转染sema3A的细胞中E-cadherin表达强烈上调，而转染sema3C的细胞中E-cadherin表达强烈下调。转染sema3A的细胞中β -连环蛋白水平未发生变化；然而，转染sema3C的细胞表达该蛋白较低。与未转染和转染sema3A的细胞相比，转染Sema3C的细胞表现出更大的细胞膜表达某些α整合素，这一特征与增加的粘附和侵袭有关。这些数据表明，sema3A和sema3C转染的PC-3细胞的侵袭能力部分与粘附蛋白的表达以及对邻近细胞成分和细胞外基质的粘附能力有关。
The class 3 semaphorins, sema3A and sema3C, provide important guidance cues in cell development and in cancer; however, the role of these semaphorins in prostate cancer is not known. We report here that sema3A transfected cells exhibit decreased invasion and adhesion in Matrigel-based assays and that sema3C transfected cells exhibit increased invasive and adhesive characteristics. Important adhesion proteins were differentially modulated in sema3A and sema3C cells in a manner consistent with their subsequent invasive and adhesive characteristics. E-cadherin expression as determined by Western blot analysis was strongly upregulated in sema3A transfected cells, but strongly downregulated in sema3C transfected cells compared to untransfected and mock empty vector-transfected PC-3 cells. beta-catenin levels were not changed in sema3A transfected cells; however, sema3C transfected cells had lower expression of this protein. Sema3C transfected cells exhibited greater cellular membrane expression of certain alpha integrins as compared to untransfected and sema3A transfected cells, a characteristic associated with increased adhesion and invasion. These data indicate that the invasive ability of sema3A and sema3C transfected PC-3 cells is, in part, correlated with adhesion protein expression and adhesive ability to constituents of neighboring cells and the extracellular matrix.