FLOW-CYTOMETRIC ENUMERATION OF MICRONUCLEATED POLYCHROMATIC ERYTHROCYTES IN MOUSE PERIPHERAL-BLOOD
FLOW-CYTOMETRIC ENUMERATION OF MICRONUCLEATED POLYCHROMATIC ERYTHROCYTES IN MOUSE PERIPHERAL-BLOOD
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DOI：
10.1002/cyto.990130711
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发表时间：
1992-01-01
期刊：
影响因子：
--
通讯作者：
AMNEUS, H
中科院分区：
文献类型：
--
作者：
GRAWE, J;ZETTERBERG, G;AMNEUS, H
描述了一种流式细胞术测定，其可用于测定小鼠外周血中微核嗜多染红细胞（PCE）和正染红细胞（NCE）的频率和DNA含量。噻唑橙子用于区分PCE和NCE，而Hoechst 33342用于检测微核PCE和NCE。在不到10分钟的时间内可以分析多达70，000个嗜多染红细胞。这相当于150- 3，000个微核嗜多染红细胞，其中90-95%是分选后用荧光显微镜确定的真实事件。在剂量反应实验中使用X射线作为诱导剂，在剂量为0.02戈伊时可记录到显著增加。该方法似乎也可能允许在比以前可能的剂量更低的剂量下检测其他诱导剂的致染色体断裂作用。
A flow-cytometric assay is described that can be used to determine the frequency and the DNA content of micro-nucleated polychromatic (PCE) and normochromatic (NCE) erythrocytes in mouse peripheral blood. Thiazole orange was used for discrimination between PCEs and NCEs, while Hoechst 33342 was used to detect micronucleated PCEs and NCEs. Up to 70,000 polychromatic erythrocytes can be analyzed in less than 10 min. This corresponds to 150-3,000 micronucleated polychromatic erythrocytes, 90-95% of which are true events as determined with a fluorescence microscope after sorting. Using X-rays as the inducing agent in dose-response experiments, a significant increase can be registered at doses of 0.02 Gy. It seems possible that the method will also allow the detection of clastogenic effects of other inducing agents at lower doses than previously possible.