Deformations of the isolated mouse tectorial membrane produced by oscillatory forces
Deformations of the isolated mouse tectorial membrane produced by oscillatory forces
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DOI：
10.1016/s0378-5955(00)00041-1
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发表时间：
2000-06-01
期刊：
影响因子：
2.8
通讯作者：
Freeman, DM
中科院分区：
文献类型：
--
作者：
Abnet, CC;Freeman, DM
通过用磁珠（半径类似于10 μ m）向TM施加振荡剪切力来测量小鼠的分离的覆膜（TM）的机械性质。正弦力在10 Hz的振幅从5到33 nN的切向施加到11个TM的表面。力与微珠位移的比值范围为0.04 - 0.98 N/m（中位数：0.18 N/m，四分位数范围：0.11-0.30 N/m，n = 90）。频率从10 Hz增加到100 Hz，磁珠位移的幅度降低了6-7.3 dB/decade。位移的相位在频率上滞后刺激电流的相位大约27-44度。邻近组织的位移随着与磁珠的距离增加而减小。空间常数为几十微米的量级。径向和纵向施加具有相等幅度和频率的力。在560对测量中，85%的纵向力引起的纵向位移是径向力引起的径向位移的1-10倍。这些结果表明，TM的以下机械性能是重要的。(1)粘弹性：TM位移的频率依赖性介于纯粘性和纯弹性材料之间，这表明两者都很重要。(2)机械耦合：空间常数表明毛束可以通过TM与相邻的毛束机械相互作用。(3)各向异性：径向的机械阻抗大于纵向。这种机械各向异性与解剖学各向异性相关，例如TM的径向取向的纤维状结构。(C)2000 Elsevier Science B. V.保留所有权利。
Mechanical properties of the isolated tectorial membrane (TM) of the mouse were measured by applying oscillatory shear forces to the TM with a magnetic bead (radius similar to 10 um). Sinusoidal forces at 10 Hz with amplitudes from 5 to 33 nN were applied tangentially to the surfaces of 11 TMs. The ratio of force to bead displacement ranged from 0.04 to 0.98 N/m (median: 0.18 N/m, interquartile range: 0.11-0.30 N/m, n = 90). Increasing frequency from 10 to 100 Hz decreased the magnitude of the displacement of the magnetic bead by 6-7.3 dB/decade. The phase of the displacement lagged that of the stimulus current by approximately 27-44 degrees across frequencies. Displacement of the adjacent tissue decreased as the distance from the magnetic bead increased. Space constants were of the order of tens of micrometers. Forces with equal amplitude and frequency were applied radially and longitudinally. Longitudinal displacements in response to longitudinal forces were 1-10 times as large as radial displacements in response to radial forces in 85% of 560 paired measurements. These results suggest that the following mechanical properties of the TM are important. (1) Viscoelasticity: The frequency dependence of TM displacement lies between that of a purely viscous and a purely elastic material, suggesting that both are important. (2) Mechanical coupling: Space constants indicate that hair bundles could interact mechanically with adjacent hair bundles via the TM. (3) Anisotropy: The mechanical impedance is greater in the radial direction than it is in the longitudinal direction. This mechanical anisotropy correlates with anatomical anisotropies, such as the radially oriented fibrillar structure of the TM. (C) 2000 Elsevier Science B.V. All rights reserved.