Non-equivalence in old- and new-flagellum daughter cells of a proliferative division in Trypanosoma brucei
Non-equivalence in old- and new-flagellum daughter cells of a proliferative division in Trypanosoma brucei
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DOI：
10.1111/mmi.14345
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发表时间：
2019-07-25
影响因子：
3.6
通讯作者：
Varga, Vladimir
中科院分区：
文献类型：
--
作者：
Abeywickrema, Movin;Vachova, Hana;Varga, Vladimir
布氏锥虫（一种有鞭毛的原生动物寄生虫）在生命周期阶段之间的分化通常通过不对称细胞分裂过程发生，产生两个形态不同的子细胞。相反，增殖细胞分裂产生两个子细胞，它们看起来相似但不相同。为了详细检查前环布氏锥虫增殖分裂的子细胞之间的差异，我们使用了最近鉴定的鞭毛连接器的成分。这些在胞质分裂过程中不对称分离，从而可以区分新鞭毛和旧鞭毛子体。我们发现两个子代之间存在明显的形态差异，特别是新鞭毛子代在 G1 早期快速而广泛地重塑。这种重塑过程涉及细胞体、鞭毛和鞭毛附着区长度的增加，并伴随着前细胞端的结构变化。老鞭毛子代经历了不同的 G1 重塑，然而，尽管如此，它们各自细胞周期的 G1 持续时间没有差异。这项工作表明，T. brucei 增殖分裂的两个子代是不等价的，并且能够对突变表型进行更精细的形态学分析。我们建议布氏锥虫和相关生物体中的所有增殖分裂都将涉及非对等性。
Differentiation of Trypanosoma brucei, a flagellated protozoan parasite, between life cycle stages typically occurs through an asymmetric cell division process, producing two morphologically distinct daughter cells. Conversely, proliferative cell divisions produce two daughter cells, which look similar but are not identical. To examine in detail differences between the daughter cells of a proliferative division of procyclic T. brucei we used the recently identified constituents of the flagella connector. These segregate asymmetrically during cytokinesis allowing the new-flagellum and the old-flagellum daughters to be distinguished. We discovered that there are distinct morphological differences between the two daughters, with the new-flagellum daughter in particular re-modelling rapidly and extensively in early G1. This re-modelling process involves an increase in cell body, flagellum and flagellum attachment zone length and is accompanied by architectural changes to the anterior cell end. The old-flagellum daughter undergoes a different G1 re-modelling, however, despite this there was no difference in G1 duration of their respective cell cycles. This work demonstrates that the two daughters of a proliferative division of T. brucei are non-equivalent and enables more refined morphological analysis of mutant phenotypes. We suggest all proliferative divisions in T. brucei and related organisms will involve non-equivalence.