Assessments of anticholinergic, antidiabetic, antioxidant activities and phenolic content of Stachys annua
Assessments of anticholinergic, antidiabetic, antioxidant activities and phenolic content of Stachys annua
复制标题
DOI：
10.1016/j.bcab.2020.101711
复制
发表时间：
2020-09-01
影响因子：
4
通讯作者：
Gulcin, Ilhami
中科院分区：
文献类型：
--
作者：
Bursal, Ercan;Taslimi, Parham;Gulcin, Ilhami
本研究测定了一年生水苏（Stachys annua）的一些生化特性，包括酚类含量、抗胆碱能、抗糖尿病和抗氧化活性。制备了一年生水苏的甲醇提取物（MESA）和水提取物（WESA），并用于所有生化分析。通过六种不同的体外生物分析方法评估了MESA和WESA的抗氧化能力，包括三种还原型抗氧化方法和三种自由基清除型抗氧化方法。此外，还分别测定了一年生水苏对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、α -淀粉酶和α -糖苷酶的酶抑制作用。结果表明，两种提取物对α -淀粉酶和α -糖苷酶均表现出较高的抑制作用，而对AChE和BChE酶的抑制作用较低。分别测定了MESA和WESA对AChE（119.8 ± 2.4μg/mL和150.1 ± 3.0μg/mL）、BChE（192.1 ± 3.8μg/mL和186.7 ± 3.7μg/mL）、α -糖苷酶（25.7 ± 0.5μg/mL和18.7 ± 0.4μg/mL）以及α -淀粉酶（43.3 ± 0.9μg/mL和11.4 ± 0.2μg/mL）的半数抑制浓度（IC50）值。本研究的另一个目的是通过液相色谱 - 串联质谱（LC - MS/MS）评估一年生水苏的酚类成分。在MESA中，鉴定出槲皮万寿菊素 - 3,6 - 二甲醚（178.6 ± 33.5μg/g）、绿原酸（118.2 ± 16.4μg/g）和富马酸（102.5 ± 7.1μg/g）为主要化合物。另一方面，在WESA中，富马酸（309.5 ± 21.5μg/g）、芹菜素（144.6 ± 11.7μg/g）和绿原酸（78.1 ± 10.8μg/g）被鉴定为主要化合物。本研究将为一年生水苏在食品或药用方面的进一步研究提供科学依据。
Some biochemical properties including phenolic content, anticholinergic, antidiabetic, and antioxidant activities of Stachys annua were determined in this study. The methanol extract of Stachys annua (MESA) and the water extract of Stachys annua (WESA) were prepared and used for all biochemical analyses. Antioxidant capacities of MESA and WESA were evaluated by six different in vitro bioanalytical methods including three reducing antioxidant methods and three radical scavenging antioxidant methods. Also, enzyme inhibition effect of Stachys annua against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), alpha-amylase, and alpha-glycosidase enzymes were determined separately. According to the results, both extracts showed high inhibition effects against alpha-amylase and alpha-glycosidase enzymes, whereas they showed low inhibition effects against AChE and BChE enzymes. The IC50 values of MESA and WESA against AChE (119.8 +/- 2.4 mu g/mL and 150.1 +/- 3.0 mu g/mL), BChE (192.1 +/- 3.8 mu g/mL and 186.7 +/- 3.7 mu g/mL), alpha-glycosidase (25.7 +/- 0.5 mu g/mL and 18.7 +/- 0.4 mu g/mL), and alpha-amylase (43.3 +/- 0.9 mu g/mL and 11.4 +/- 0.2 mu g/mL) were determined, respectively. Another goal of the study was to evaluate the phenolic compositions of Stachys annua by liquid chromatography and tandem mass spectrometry (LC-MS/MS). Quercetagetin-3,6-dimethylether (178.6 +/- 33.5 mu g/g), chlorogenic acid (118.2 +/- 16.4 mu g/g), and fumaric acid (102.5 +/- 7.1 mu g/g) were identified as major compounds in MESA. On the other hand, fumaric acid (309.5 +/- 21.5 mu g/g), apigenin (144.6 +/- 11.7 mu g/g), and chlorogenic acid (78.1 +/- 10.8 mu g/g) were identified as major compounds in WESA. This study will be a scientific base for further studies about Stachys annua for food or medicinal utilization.