Transcriptome analysis ofleafynear-isogenic lines provides molecular insights into floral transition in maize (Zea mays)
Transcriptome analysis ofleafynear-isogenic lines provides molecular insights into floral transition in maize (Zea mays)
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叶近等基因系的转录组分析提供了玉米花转变的分子见解
DOI：
10.1111/pbr.12849
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发表时间：
2020-07-29
期刊：
影响因子：
2
通讯作者：
Wang, Guoying
中科院分区：
文献类型：
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作者：
Du, Xuemei;Wang, Xiaoli;Wang, Guoying
LEAFY(Lfy1)是一个典型的晚花突变体，其基因座已被精细定位，但其潜在的分子机制尚不清楚。在本研究中，我们培育了两个带有和不带有Lfy1基因座的近等基因系，用于Lfy1介导的花转换的表型和转录分析。表型观察表明，野生型(WT)植株在V6(6片展开叶)阶段进入雄穗原基，而Lfy1突变体一直维持营养生长至V9阶段。转录组分析显示，两个品系的茎尖在V5期(394个)比V3期(227个)有更多的差异表达基因(Deg)。功能浓缩分析表明，在V5期，生物钟通路基因在Lfy1突变体中的表达水平低于在WT中的表达水平。一些作为生物钟基因下游调控基因的整合子基因在突变系中也表现出较低的转录丰度，这表明Lfy1介导的晚花表型部分是由于生物钟途径基因的低效表达造成的。这些结果为Lfy1突变体的晚花表型提供了分子线索。
Leafy(Lfy1) is a typical late-flowering mutant with locus being fine-mapped, but its underlying molecular mechanism is unknown. In this study, two near-isogenic lines, with and without theLfy1locus, were developed for phenotypic and transcriptional analysis ofLfy1-mediated floral transition. Phenotypic observation showed that wild-type (WT) plants transited into the tassel primordium at the V6 (6 expanded leaves) stage, whereas theLfy1mutant maintained the vegetative stage up to the V9 stage. Transcriptome analysis of shoot tips of both lines revealed more differentially expressed genes (DEGs) at the V5 (394) than the V3 stage (227). Functional enrichment analysis showed that circadian clock pathway genes exhibited lower expression levels in theLfy1mutant than in the WT at the V5 stage. A few integrator genes that functioned as downstream regulators of circadian clock genes also showed a lower transcript abundance in mutant lines, suggesting that theLfy1-mediated late-flowering phenotype was partially caused by inefficient expression of circadian clock pathway genes. These results provide molecular clues for the late-flowering phenotype of theLfy1mutant.