Notch expression patterns in the retina: An eye on receptor-ligand distribution during angiogenesis
Notch expression patterns in the retina: An eye on receptor-ligand distribution during angiogenesis
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DOI：
10.1016/j.modgep.2006.11.002
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发表时间：
2007-02-01
影响因子：
1.2
通讯作者：
Iruela-Arispe, M. Luisa
中科院分区：
文献类型：
--
作者：
Hofmann, Jennifer J.;Iruela-Arispe, M. Luisa
Notch信号通路对血管形态发生的重要贡献已经通过小鼠和斑马鱼的功能丧失研究得到了强调。然而，对这一信号系统如何在细胞和分子水平上影响血管形成的全面理解还远远没有达到。在此，我们详细分析了活性Notch1在血管形态发生和成熟的进展阶段与其DSL （Delta, Serrate, Lag2）配体，Jagged1， Delta-like1和Delta-like4的分布。发现Notch配体的细胞分布存在重要差异。Jagged1 （Jag1）在主要脉管系统的“柄细胞”和动脉分支点检测到，而Delta-like4 （D114）明显缺失。D114是维管芽生长末端“尖端细胞”中唯一表达的配体。在成熟动脉的茎细胞、毛细血管、动脉内皮和壁细胞中也均存在。在发育网络的动脉和静脉中均观察到delta -样1 (D111)，但在成熟的动脉分支点中也被排除在外。这些发现支持Notch配体在诱导血管生成过程中的不同作用。(c) 2006 Elsevier B.V.版权所有
The critical contribution of the Notch signaling pathway to vascular morphogenesis has been underscored by loss-of-function studies in mouse and zebrafish. Nonetheless, a comprehensive understanding as to how this signaling system influences the formation of blood vessels at the cellular and molecular level is far from reached. Here, we provide a detailed analysis of the distribution of active Notch1 in relation to its DSL (Delta, Serrate, Lag2) ligands, Jagged1, Delta-like1, and Delta-like4, during progressive stages of vascular morphogenesis and maturation. Important differences in the cellular distribution of Notch ligands were found. Jagged1 (Jag1) was detected in "stalk cells" of the leading vasculature and at arterial branch points, a site where Delta-like4 (D114) was clearly absent. D114 was the only ligand expressed in "tip cells" at the end of the growing vascular sprouts. It was also present in stalk cells, capillaries, arterial endothelium, and in mural cells of mature arteries in a homogenous manner. Delta-like 1 (D111) was observed in both arteries and veins of the developing network, but was also excluded from mature arterial branch points. These findings support alternative and distinct roles for Notch ligands (luring the angiogenic process. (c) 2006 Elsevier B.V. All rights reserved.