Different properties between spontaneous and volume-activated chloride currents in human nasopharyngeal carcinoma and its normal counterpart cells
Different properties between spontaneous and volume-activated chloride currents in human nasopharyngeal carcinoma and its normal counterpart cells
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人鼻咽癌及其正常对应细胞自发和容量激活氯电流的不同特性
DOI：
10.1002/cbf.3419
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发表时间：
2019-08-01
影响因子：
3.6
通讯作者：
Zhu，Linyan
中科院分区：
文献类型：
--
作者：
Tang，Xinwei;Feng，Jiezhu;Zhu，Linyan
尽管自发氯电流（SCC）在正常细胞中已得到充分研究，但其在肿瘤细胞中的特性和作用仍不清楚。在这里，我们发现低分化的人鼻咽癌CNE-2Z细胞中出现了SCC，与体积激活氯电流（VACC）相比，存在一些差异，例如发生率较低和电流密度更大。 NPPB 是一种氯通道阻滞剂，对 SCC 的抑制作用比 VACC 强得多。氯离子通道 ‐3 (ClC-3)（一种体积和机械依赖性离子通道）的下调可以显着降低 VACC，但在 SCC 中则不然。正常鼻咽 NP69-SV40T 细胞中 SCC 的发生率、潜伏期和平均密度均远低于 CNE-2Z 细胞。我们的研究结果表明，肿瘤细胞的自发电反应性高于正常细胞，这可能与肿瘤细胞的高生理活性有关。研究意义自发氯电流（SCC）在兴奋组织中众所周知，调节多种生理和病理生理过程。在我们对人鼻咽癌CNE-2Z细胞的容量激活氯电流（VACC）进行研究时，也可以观察到具有与VACC不同特性的SCC。同时，CNE-2Z 细胞中 SCC 的发生率、潜伏期和平均密度远高于正常鼻咽 NP69-SV40T 细胞。我们的研究结果揭示了SCC在癌细胞中的表达和特征，为进一步探索SCC在肿瘤细胞生物学中的功能提供了初步的实验基础。
Although the spontaneous chloride currents (SCC) have been well studied in the normal cells, its properties and roles in neoplasms cells are still unknown. Here, we found that the SCC was manifested in the poorly differentiated human nasopharyngeal carcinoma CNE‐2Z cells, with some differences such as lower occurrence and bigger current density than those of the volume‐activated chloride currents (VACC). NPPB, a chloride channel blocker, inhibited the SCC much stronger than the VACC. Down‐regulation of chloride channel ‐3 (ClC‐3), a volume and mechanically dependent ion channel, could significantly decrease the VACC, but not in SCC. The occurrence, latency, and mean density of the SCC were much lower in the normal nasopharyngeal NP69‐SV40T cells than those in CNE‐2Z cells. Our results demonstrated that the spontaneous electrical reactivity of neoplasm cells is higher than that of normal cells, which probably relates to their high physiological activity of neoplasm cells.Significance of the studySpontaneous chloride currents (SCC) are well known in excitable tissues and regulate a variety of physiological and pathophysiological processes. During our researching on the volume‐activated chloride currents (VACC) in human nasopharyngeal carcinoma CNE‐2Z cells, SCC could be also observed with different properties from VACC. Meanwhile, the occurrence, latency, and mean density of the SCC were much higher in CNE‐2Z cells than those in normal nasopharyngeal NP69‐SV40T cells. Our results revealed the expression and characteristics of SCC in carcinoma cells and provided a preliminary experimental basis for further exploring the function of SCC in tumour cell biology.