The AreA Nitrogen Catabolite Repression Activator Balances Fungal Nutrient Utilization and Virulence in the Insect Fungal Pathogen Beauveria bassiana.
The AreA Nitrogen Catabolite Repression Activator Balances Fungal Nutrient Utilization and Virulence in the Insect Fungal Pathogen Beauveria bassiana.
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DOI：
10.1021/acs.jafc.2c07047
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发表时间：
2022-12
影响因子：
6.1
通讯作者：
Zhibing Luo;Qiyan Chen;Yufeng Su;Shasha Hu;N. Keyhani;Junyao Wang;Chenhua Zhu;Teng Zhou;Yunxia Pan;M. Bidochka;Yongjun Zhang
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文献类型：
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作者：
Zhibing Luo;Qiyan Chen;Yufeng Su;Shasha Hu;N. Keyhani;Junyao Wang;Chenhua Zhu;Teng Zhou;Yunxia Pan;M. Bidochka;Yongjun Zhang
在许多真菌中，AreA GATA 型转录因子介导氮分解代谢物抑制，影响真菌发育，并在适用的情况下影响毒力。在这里，我们通过基因表达的敲低研究了 AreA 在真菌昆虫病原体和植物内生白僵菌中的功能。 antiAreA 突变体的氮利用受损，对渗透应激源的敏感性增加，但对氧化/缺氧应激的耐受性增加。 BbAreA 的抑制对真菌毒力的总体影响很小。对毒力的轻微影响似乎部分归因于竞争性次级效应，其中宿主防御酚氧化酶活性显着降低，但真菌代谢物卵孢子蛋白的产生增加且菌丝体发育受损。 BbAreA 表达的敲低也会导致真菌与宿主植物结合的能力受损。这些数据表明，BbAreA 可能充当平衡真菌营养利用、致病性和互利共生的调节剂，促进真菌占据宿主生态位。
In many fungi, the AreA GATA-type transcription factor mediates nitrogen catabolite repression affecting fungal development and, where applicable, virulence. Here, we investigated the functions of AreA in the fungal entomopathogen and plant endophyte Beauveria bassiana using knockdown of gene expression. The antiAreA mutants were impaired in nitrogen utilization and showed increased sensitivities to osmotic stressors but increased tolerances to oxidative/hypoxia stresses. Repression of BbAreA caused overall minimal effects on fungal virulence. The minor effects on virulence appeared to be due in part to competing secondary effects where host defense phenoloxidase activity was significantly decreased, but production of the fungal metabolite oosporein was increased and hyphal body development was impaired. Knockdown of BbAreA expression also resulted in impairment in ability of the fungus to associate with host plants. These data implicate that BbAreA likely acts as a regulator to balance fungal nutrient utilization, pathogenicity, and mutualism, facilitating the fungal occupation of host niches.