Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients
Elevated levels of phosphorylated fibrinogen-α-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients
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DOI：
10.1021/pr060344
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发表时间：
2006-12-01
影响因子：
4.4
通讯作者：
Muddiman, David C.
中科院分区：
文献类型：
--
作者：
Ogata, Yuko;Hepplmann, Carrie J.;Muddiman, David C.
我们使用2-D SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）和翻译后修饰（PTM）特异性染色，在去除6个高丰度蛋白后，评估卵巢癌（OVC）患者血浆中差异表达的蛋白质。将III期或IV期OVC患者的合并血浆与绝经后年龄匹配的合并对照进行比较。几种蛋白质被鉴定为在OVC患者的血浆中差异表达。其中，发现磷酸化纤维蛋白原-α-链同种型（含有纤维蛋白肽-A）被上调。以前在我们的实验室中，磷酸化纤维蛋白肽-A被发现在来自OVC患者的血清的低分子量部分中上调。我们使用蛋白质印迹、质谱（MS）和PTM特异性染色检测了构成合并血浆的每例患者中磷酸化纤维蛋白原-α-链的水平。在所有OVC患者中，含有纤维蛋白原-α-链片段的磷蛋白条带显示上调。
We evaluated the differentially expressed proteins in the plasma of ovarian cancer (OVC) patients using 2-D SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with post-translational modification (PTM) specific stains after the removal of six high-abundance proteins. The pooled plasma from patients with stage III or IV OVC was compared to a pooled postmenopausal age-matched control. Several proteins were identified as differentially expressed in the plasma of OVC patients. Among them, the phosphorylated fibrinogen-alpha-chain isoform (containing fibrinopeptide-A) was found to be up-regulated. Previously in our laboratory, phosphorylated fibrinopeptide-A was found to be up-regulated in the low molecular weight fraction of serum derived from OVC patients. We examined the levels of phosphorylated fibrinogen-alpha-chain in each patient that constituted the pooled plasma using Western blot, mass spectrometry (MS), and PTM specific stains. Phosphoprotein bands containing fibrinogen-alpha-chain fragments showed up-regulation in all OVC patients.