Nontransducing rhodopsin.
Nontransducing rhodopsin.
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非转导视紫红质。
DOI：
10.1085/jgp.90.4.575
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Lisman,J
中科院分区：
文献类型：
--
作者：
Levine,E;Crain,E;Robinson,P;Lisman,J
视紫红质被光转化为触发转导级联的活性光产物。然后必须通过某种化学修饰使活性光产物失活。这里要解决的问题是，非活性光产物向视紫红质的光转化是否产生了不能支持转导的视紫红质的修饰形式。这个问题进行了研究，在紫外线受体的鲎中眼通过测量激发后的相对量子效率的光再生的视紫红质从非活性的光产物。结果表明，当这种新产生的视紫红质吸收光子时，没有受体电位产生;即，色素是非转导的。一个黑暗的过程需要30-60分钟，使视紫红质恢复到其转导形式。
Rhodopsin is converted by light to an active photoproduct that triggers the transduction cascade. The active photoproduct must then be inactivated by some kind of chemical modification. The question addressed here is whether photoconversion of the inactive photoproduct to rhodopsin creates a modified form of rhodopsin that is unable to support transduction. This question was investigated in ultraviolet receptors of Limulus median eye by measuring the relative quantum efficiency of excitation after photoregeneration of rhodopsin from the inactive photoproduct. The results show that when this newly created rhodopsin absorbs a photon, no receptor potential is generated; ie, the pigment is nontransducing. A dark process requiring 30-60 rain returns rhodopsin to its transducing form.