A novel regulatory mechanism of naringenin through inhibition of T lymphocyte function in contact hypersensitivity suppression
A novel regulatory mechanism of naringenin through inhibition of T lymphocyte function in contact hypersensitivity suppression
复制标题
柚皮素通过抑制 T 淋巴细胞功能来抑制接触性超敏反应的新型调节机制。
DOI：
10.1016/j.bbrc.2010.05.065
复制
发表时间：
2010-06-25
影响因子：
3.1
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
--
作者：
Fang, Feng;Tang, Yijun;Xu, Qiang
柚皮素是葡萄柚和柑橘类水果中的一种类黄酮，据报道具有抗炎和抗氧化活性。接触性超敏反应（CHS）是一种T细胞介导的免疫反应，巨噬细胞释放的因子也参与了这种反应。既往研究表明柚皮素通过抑制巨噬细胞的活化和迁移来抑制CHS。然而，对柚皮素对T淋巴细胞的作用知之甚少。我们的研究表明柚皮素通过抑制T淋巴细胞的增殖和活化来有效抑制苦味醇氯（PCI）诱导的接触性超敏反应。在体外实验中，柚皮素处理后，活化的半抗原特异性T细胞和抗cd3 /抗cd28刺激的T细胞均出现生长停滞。此外，柚皮素降低了CD69（蛋白质水平）和细胞因子如IL-2、tnf - α和ifn - γ （mRNA水平）的表达，这些细胞因子在活化的T细胞中高度表达。同时，柚皮素还通过上调Bax、Bad、PARP、cleaved-caspase 3和下调磷酸化Akt、Bcl-2诱导T细胞凋亡。上述结果提示，柚皮素除对巨噬细胞具有抗炎作用外，还具有抑制T细胞活化和增殖的作用，可缓解接触性超敏反应症状。(C) 2010爱思唯尔公司版权所有。
Naringenin, a flavonoid in grapefruits and citrus fruits, has been reported to exhibit anti-inflammatory and anti-oxidative activities. Contact hypersensitivity (CHS) is a T cell-mediated immune reaction, and the factors released from macrophages also contribute to this response. Previous studies showed that naringenin suppressed CHS by inhibiting activation and migration of macrophages. However, little is known about naringenin's effects on T lymphocytes. Our study indicated that naringenin potently suppressed picryl chloride (PCI)-induced contact hypersensitivity by inhibiting the proliferation and activation of T lymphocytes. in vitro, both of the activated hapten-specific T cells and the T cells stimulated with anti-CD3/anti-CD28 showed growth arrest after naringenin treatment. Furthermore, naringenin reduced CD69 (the protein level) and cytokines such as IL-2, TNF-alpha, and IFN-gamma (the mRNA level) expressions which highly expressed by activated T cells. Meanwhile, naringenin also induced T cell apoptosis by upregulation of Bax, Bad, PARP, cleaved-caspase 3 and downregulation of phosphorylated Akt, Bcl-2. These findings suggest that, besides its anti-inflammatory activities in macrophages, naringenin also showed inhibitory effects on the activation and proliferation of T cells to alleviate symptoms of contact hypersensitivity. (C) 2010 Elsevier Inc. All rights reserved.