Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae.
Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae.
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DOI：
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发表时间：
1996-03
期刊：
影响因子：
3.3
通讯作者：
H. Fan;Kenneth K. Cheng;H. Klein
中科院分区：
文献类型：
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作者：
H. Fan;Kenneth K. Cheng;H. Klein
soh 1，soh 2和soh 4突变体被分离作为抑制剂的温度依赖性生长的超重组突变体hpr 1的酿酒酵母。这些抑制基因的克隆和序列分析出乎意料地显示它们编码RNA聚合酶II转录复合物的组分。SOH 2与RPB 2相同，编码RNA聚合酶II的第二大亚基，SOH 4与SUA 7相同，编码酵母转录起始因子TFIIB。SOH 1编码一种新的14 kD蛋白，与RNA聚合酶具有有限的序列相似性。有趣的是，SOH 1不仅与参与DNA修复的因子相互作用，还与转录因子相互作用。因此，Soh 1蛋白可以用于耦合这两个过程。Soh 1蛋白在双杂交系统测定中与DNA修复蛋白Rad 5 p相互作用。Soh 1 p可能与RNA聚合酶II复合物的组分在功能上相互作用，这从在soh 1 rpb delta 104、soh 1 soh 2 -1（rpb 2）和soh 1 soh 4（sua 7）双突变体中观察到的合成致死性中得到了证实。由于SOH 1、RPB 2和SUA 7突变抑制了hpr 1突变体的超重组表型，这表明了同向重复序列中的重组与转录之间的联系。
The soh1, soh2 and soh4 mutants were isolated as suppressors of the temperature-dependent growth of the hyperrecombination mutant hpr1 of Saccharomyces cerevisiae. Cloning and sequence analysis of these suppressor genes has unexpectedly shown them to code for components of the RNA polymerase II transcription complex. SOH2 is identical to RPB2, which encodes the second largest subunit of RNA polymerase II, and SOH4 is the same as SUA7, encoding the yeast transcription initiation factor TFIIB. SOH1 encodes a novel 14-kD protein with limited sequence similarity to RNA polymerases. Interestingly, SOH1 not only interacts with factors involved in DNA repair, but transcription as well. Thus, the Soh1 protein may serve to couple these two processes. The Soh1 protein interacts with a DNA repair protein, Rad5p, in a two-hybrid system assay. Soh1p may functionally interact with components of the RNA polymerase II complex as suggested from the synthetic lethality observed in soh1 rpb delta 104, soh1 soh2-1 (rpb2), and soh1 soh4 (sua7) double mutants. Because mutations in SOH1, RPB2 and SUA7 suppress the hyperrecombination phenotype of hpr1 mutants, this suggests a link between recombination in direct repeats and transcription.